Linked Life Data

12662902

Source:http://linkedlifedata.com/resource/pubmed/id/12662902

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-3-28
pubmed:abstractText
Sulfotransferases (STs) catalyze the sulfation of many structurally diverse molecules. Enzymatic assays and Western blots have been used to identify and characterize STs in the human gastrointestinal tract. Sulfation activities for 2-naphthol, dopamine, estradiol, and dehydroepiandrosterone (DHEA) from 23 donors were measured in cytosol prepared from stomach, duodenum, segments of small intestine, and colon and were compared to levels in human liver cytosol. Stomach and colon had low 2-naphthol and dopamine sulfation activities and almost no estradiol and DHEA sulfation activity. For all four substrates, small intestine has higher activities than both stomach and colon. Human small intestine 2-naphthol sulfation specific activity is approximately half that of human liver. Human small intestine dopamine sulfation activity is three times as high as that of human liver. While estrogen sulfation activity is about the same for both human intestine and human liver, human liver DHEA sulfation activity is about five times as high as that of human small intestine. The distribution of ST activities along the length of the small intestine was very different among different donors. Some donors had higher activity in the proximal segments of the small intestine, whereas other donors had higher activity in the distal segments of the small intestine. Our results also demonstrated high variation of small intestine sulfation activities compared with human liver activities among different donors. The Western blot results agreed with the enzymatic assay results. These results suggest that xenobiotics may regulate human small intestinal STs.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0041-008X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
187
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
186-97
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12662902-Adolescent, pubmed-meshheading:12662902-Adult, pubmed-meshheading:12662902-Aged, pubmed-meshheading:12662902-Blotting, Western, pubmed-meshheading:12662902-Catalysis, pubmed-meshheading:12662902-Child, pubmed-meshheading:12662902-Colon, pubmed-meshheading:12662902-Cytosol, pubmed-meshheading:12662902-Dehydroepiandrosterone, pubmed-meshheading:12662902-Digestive System, pubmed-meshheading:12662902-Dopamine, pubmed-meshheading:12662902-Estradiol, pubmed-meshheading:12662902-Female, pubmed-meshheading:12662902-Humans, pubmed-meshheading:12662902-Intestine, Small, pubmed-meshheading:12662902-Male, pubmed-meshheading:12662902-Middle Aged, pubmed-meshheading:12662902-Naphthols, pubmed-meshheading:12662902-Phosphoadenosine Phosphosulfate, pubmed-meshheading:12662902-Protein Isoforms, pubmed-meshheading:12662902-Sulfotransferases, pubmed-meshheading:12662902-Tritium
pubmed:year
2003
pubmed:articleTitle
Human gastrointestinal sulfotransferases: identification and distribution.
pubmed:affiliation
Department of Physiological Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA. cguang@okstate.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.