Source:http://linkedlifedata.com/resource/pubmed/id/12660412
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2003-3-27
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| pubmed:abstractText |
In order to develop a model in mouse similar to anti- Thy-1 nephritis in the rat, we prepared sheep antiserum against SV40-transformed mouse mesangial (MES 13) cells. In vivo, the anti-mouse mesangial cell serum-treated mice showed severe azotemia that peaked at day 6 and proteinuria that peaked at day 8, in a dose-dependent fashion. Light microscopy and electron microscopy showed duplication of glomerular basement membranes, mesangiolysis, subendothelial and mesangial electron-dense deposits, and foot process effacement. Intraglomerular tuft cell number was significantly reduced at day 4 and there were increased numbers of apoptotic cells at days 2 and 4. SCID mice and mice lacking C3 manifested similar responses to anti-mouse mesangial cell serum, suggesting that T cells, B cells and complement are not required for glomerular injury in this model. In vitro, anti-mouse mesangial cell serum treated mesangial cells showed greater release of lactate dehydrogenase, decreased cell survival, and increased apoptotic cell death. Anti-mouse mesangial cell serum induces glomerulopathy characterized by mesangiolysis and mesangial cell apoptosis, and followed by cellular proliferation.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1660-2129
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2003 S. Karger AG, Basel
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| pubmed:issnType |
Electronic
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| pubmed:volume |
93
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
e92-106
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| pubmed:dateRevised |
2003-11-14
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| pubmed:meshHeading |
pubmed-meshheading:12660412-3T3 Cells,
pubmed-meshheading:12660412-Acute Disease,
pubmed-meshheading:12660412-Animals,
pubmed-meshheading:12660412-Antibody Specificity,
pubmed-meshheading:12660412-Antigens, Surface,
pubmed-meshheading:12660412-Apoptosis,
pubmed-meshheading:12660412-Cell Division,
pubmed-meshheading:12660412-Cell Line,
pubmed-meshheading:12660412-Disease Models, Animal,
pubmed-meshheading:12660412-Dose-Response Relationship, Immunologic,
pubmed-meshheading:12660412-Endotoxins,
pubmed-meshheading:12660412-Female,
pubmed-meshheading:12660412-Glomerular Mesangium,
pubmed-meshheading:12660412-Glomerulonephritis, Membranous,
pubmed-meshheading:12660412-Immune Sera,
pubmed-meshheading:12660412-Immunoglobulins,
pubmed-meshheading:12660412-Kidney,
pubmed-meshheading:12660412-Lung,
pubmed-meshheading:12660412-Mice,
pubmed-meshheading:12660412-Mice, Congenic,
pubmed-meshheading:12660412-Mice, Inbred BALB C,
pubmed-meshheading:12660412-Mice, Inbred Strains,
pubmed-meshheading:12660412-Mice, SCID,
pubmed-meshheading:12660412-Organ Specificity,
pubmed-meshheading:12660412-Sheep,
pubmed-meshheading:12660412-Time Factors
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| pubmed:year |
2003
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| pubmed:articleTitle |
Anti-mouse mesangial cell serum induces acute glomerulonephropathy in mice.
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| pubmed:affiliation |
Kidney Disease Section, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892-1268, USA.
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| pubmed:publicationType |
Journal Article
|