Source:http://linkedlifedata.com/resource/pubmed/id/12660142
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2003-7-9
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| pubmed:abstractText |
Surfactant-like particles (SLP) are unilamellar secreted membranes associated with the process of lipid absorption and isolated previously only from the apical surface of enterocytes. In this paper, the intracellular membrane has been isolated from corn oil-fed animals, identified by its content of the marker protein intestinal alkaline phosphatase (IAP). Another brush-border protein, cubilin, and its anchoring protein megalin have been identified as components of extracellular SLP, but only cubilin is present to any extent in intracellular SLP. During fat absorption, IAP is modestly enriched in intracellular SLP, but full-length cubilin (migrating at 210 kDa in fat-fed mucosal fractions) falls by one-half, although fragments of cubilin are abundant in the intracellular SLP. Both IAP and cubilin colocalize to the same cells during corn oil absorption and colocalize around lipid droplets. This localization is more intense during feeding of corn oil with Pluronic L-81, a detergent that allows uptake of fatty acids and monoglycerides from the lumen, but blocks chylomicron secretion. Confocal microscopy confirms the colocalization of IAP and the ligand for cubilin, intrinsic factor. Possible roles for cubilin in intracellular SLP include facilitating movement of the lipid droplet through the cell and binding to the basolateral membrane before reverse endocytosis.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Corn Oil,
http://linkedlifedata.com/resource/pubmed/chemical/Dietary Fats, Unsaturated,
http://linkedlifedata.com/resource/pubmed/chemical/Intrinsic Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Low Density Lipoprotein...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Surface-Active Agents,
http://linkedlifedata.com/resource/pubmed/chemical/intrinsic factor-cobalamin receptor
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0193-1857
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
285
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
G433-41
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:12660142-Alkaline Phosphatase,
pubmed-meshheading:12660142-Animals,
pubmed-meshheading:12660142-Corn Oil,
pubmed-meshheading:12660142-Dietary Fats, Unsaturated,
pubmed-meshheading:12660142-Endocytosis,
pubmed-meshheading:12660142-Enterocytes,
pubmed-meshheading:12660142-Intestinal Mucosa,
pubmed-meshheading:12660142-Intestines,
pubmed-meshheading:12660142-Intracellular Membranes,
pubmed-meshheading:12660142-Intrinsic Factor,
pubmed-meshheading:12660142-Low Density Lipoprotein Receptor-Related Protein-2,
pubmed-meshheading:12660142-Male,
pubmed-meshheading:12660142-Microscopy, Confocal,
pubmed-meshheading:12660142-Microscopy, Electron,
pubmed-meshheading:12660142-Rats,
pubmed-meshheading:12660142-Rats, Sprague-Dawley,
pubmed-meshheading:12660142-Receptors, Cell Surface,
pubmed-meshheading:12660142-Surface-Active Agents
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| pubmed:year |
2003
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| pubmed:articleTitle |
Rat enterocytes secrete SLPs containing alkaline phosphatase and cubilin in response to corn oil feeding.
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| pubmed:affiliation |
Dept. of Medicine, Washington Univ. School of Medicine, 660 S. Euclid Ave., St. Louis, MO 63110-1010, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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