Source:http://linkedlifedata.com/resource/pubmed/id/12659216
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2003-3-27
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pubmed:abstractText |
Capillary electrophoresis/electrospray ionization-mass spectrometry (CE/ESI-MS) was applied to the analysis of underivatized amino acids and the separation of their D/L-enantiomers. Under full-scan mode, all standard protein amino acids were separated and detected at low-femtomole levels using a 130-cm-long, 20-microm-i.d., 150-microm-o.d. underivatized fused-silica capillary with 1 M formic acid as the background electrolyte. The CE/ESI-MS technique was also applied to the separation of L-arginine from L-canavanine (a close analogue of arginine where the terminal methylene linked to the guanidine group of arginine is replaced by an oxygen atom) in a complex mixture containing all standard protein amino acids. The utility of CE/ESI-MS in the analysis of real-world samples was demonstrated by the identification of two metabolic diseases (PKU and tyrosinemia) through blood analysis with minimal sample preparation. In addition, the on-line separation of 11 underivatized L-amino acids from their D-enantiomers was achieved by using a 30 mM solution of (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid as the background electrolyte.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0003-2700
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1508-13
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:12659216-Amino Acids,
pubmed-meshheading:12659216-Arginine,
pubmed-meshheading:12659216-Canavanine,
pubmed-meshheading:12659216-Electrophoresis, Capillary,
pubmed-meshheading:12659216-Humans,
pubmed-meshheading:12659216-Infant, Newborn,
pubmed-meshheading:12659216-Phenylketonurias,
pubmed-meshheading:12659216-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:12659216-Stereoisomerism,
pubmed-meshheading:12659216-Tyrosinemias
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pubmed:year |
2003
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pubmed:articleTitle |
Analysis of underivatized amino acids and their D/L-enantiomers by sheathless capillary electrophoresis/electrospray ionization mass spectrometry.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA.
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pubmed:publicationType |
Journal Article
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