Linked Life Data

12657693

Source:http://linkedlifedata.com/resource/pubmed/id/12657693

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-3-26
pubmed:abstractText
In addition to genomic pathways, estrogens may regulate gene expression by activating specific signal transduction pathways, such as that involving phosphatidylinositol 3-kinase (PI3-K) and the subsequent phosphorylation of Akt (protein kinase B). The Akt pathway regulates various cellular events, including the initiation of protein synthesis. Our previous studies showed that synaptogenesis in hippocampal CA1 pyramidal cell dendritic spines is highest when brain estrogen levels are highest. To address the role of Akt in this process, the subcellular distribution of phosphorylated Akt immunoreactivity (pAkt-I) in the hippocampus of female rats across the estrous cycle and male rats was analyzed by light microscopy (LM) and electron microscopy (EM). By LM, the density of pAkt-I in stratum radiatum of CA1 was significantly higher in proestrus rats (or in estrogen-supplemented ovariectomized females) compared with diestrus, estrus, or male rats. By EM, pAkt-I was found throughout the shafts and in select spines of stratum radiatum dendrites. Quantitative ultrastructural analysis identifying pAkt-I with immunogold particles revealed that proestrus rats compared with diestrus, estrus, and male rats contained significantly higher pAkt-I associated with (1) dendritic spines (both cytoplasm and plasmalemma), (2) spine apparati located within 0.1 microm of dendritic spine bases, (3) endoplasmic reticula and polyribosomes in the cytoplasm of dendritic shafts, and (4) the plasmalemma of dendritic shafts. These findings suggest that estrogens may regulate spine formation in CA1 pyramidal neurons via Akt-mediated signaling events.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2340-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:12657693-Animals, pubmed-meshheading:12657693-Dendrites, pubmed-meshheading:12657693-Diestrus, pubmed-meshheading:12657693-Estradiol, pubmed-meshheading:12657693-Estrogens, pubmed-meshheading:12657693-Estrus, pubmed-meshheading:12657693-Female, pubmed-meshheading:12657693-Hippocampus, pubmed-meshheading:12657693-Immunohistochemistry, pubmed-meshheading:12657693-Male, pubmed-meshheading:12657693-Microscopy, Electron, pubmed-meshheading:12657693-Ovariectomy, pubmed-meshheading:12657693-Phosphorylation, pubmed-meshheading:12657693-Proestrus, pubmed-meshheading:12657693-Protein-Serine-Threonine Kinases, pubmed-meshheading:12657693-Proto-Oncogene Proteins, pubmed-meshheading:12657693-Proto-Oncogene Proteins c-akt, pubmed-meshheading:12657693-Pyramidal Cells, pubmed-meshheading:12657693-Rats, pubmed-meshheading:12657693-Rats, Sprague-Dawley, pubmed-meshheading:12657693-Sex Factors
pubmed:year
2003
pubmed:articleTitle
Estrogen levels regulate the subcellular distribution of phosphorylated Akt in hippocampal CA1 dendrites.
pubmed:affiliation
Division of Neurobiology, Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, New York 10021, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't