| pubmed-article:12657690 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12657690 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:12657690 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:12657690 | lifeskim:mentions | umls-concept:C0439849 | lld:lifeskim |
| pubmed-article:12657690 | lifeskim:mentions | umls-concept:C1704387 | lld:lifeskim |
| pubmed-article:12657690 | lifeskim:mentions | umls-concept:C1521816 | lld:lifeskim |
| pubmed-article:12657690 | lifeskim:mentions | umls-concept:C1522642 | lld:lifeskim |
| pubmed-article:12657690 | lifeskim:mentions | umls-concept:C0314603 | lld:lifeskim |
| pubmed-article:12657690 | lifeskim:mentions | umls-concept:C0679199 | lld:lifeskim |
| pubmed-article:12657690 | lifeskim:mentions | umls-concept:C0205464 | lld:lifeskim |
| pubmed-article:12657690 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:12657690 | pubmed:dateCreated | 2003-3-26 | lld:pubmed |
| pubmed-article:12657690 | pubmed:abstractText | Analysis of cellular morphology is the most general approach to neuronal classification. With the increased use of genetically engineered mice, there is a growing need for methods that can selectively visualize the morphologies of specified subsets of neurons. This capability is needed both to define cell morphologic phenotypes and to mark cells in a noninvasive manner for lineage studies. To this end, we describe a bipartite genetic system based on a Cre-estrogen receptor (ER) fusion protein that irreversibly activates a plasma membrane-bound alkaline phosphatase reporter gene by site-specific recombination. Because the efficiency and timing of gene rearrangement is controlled pharmacologically, a sparse subset of labeled cells can be generated from the set of CreER-expressing cells at any time during development. Histochemical visualization of alkaline phosphatase activity reveals neuronal morphology with strong and uniform labeling of all processes. | lld:pubmed |
| pubmed-article:12657690 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12657690 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12657690 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12657690 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12657690 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:12657690 | pubmed:issn | 1529-2401 | lld:pubmed |
| pubmed-article:12657690 | pubmed:author | pubmed-author:WangYanshuY | lld:pubmed |
| pubmed-article:12657690 | pubmed:author | pubmed-author:NathansJeremy... | lld:pubmed |
| pubmed-article:12657690 | pubmed:author | pubmed-author:BadeaTudor... | lld:pubmed |
| pubmed-article:12657690 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:12657690 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:12657690 | pubmed:volume | 23 | lld:pubmed |
| pubmed-article:12657690 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12657690 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12657690 | pubmed:pagination | 2314-22 | lld:pubmed |
| pubmed-article:12657690 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:12657690 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12657690 | pubmed:articleTitle | A noninvasive genetic/pharmacologic strategy for visualizing cell morphology and clonal relationships in the mouse. | lld:pubmed |
| pubmed-article:12657690 | pubmed:affiliation | Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA. | lld:pubmed |
| pubmed-article:12657690 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12657690 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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