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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2003-3-26
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pubmed:abstractText |
Analysis of cellular morphology is the most general approach to neuronal classification. With the increased use of genetically engineered mice, there is a growing need for methods that can selectively visualize the morphologies of specified subsets of neurons. This capability is needed both to define cell morphologic phenotypes and to mark cells in a noninvasive manner for lineage studies. To this end, we describe a bipartite genetic system based on a Cre-estrogen receptor (ER) fusion protein that irreversibly activates a plasma membrane-bound alkaline phosphatase reporter gene by site-specific recombination. Because the efficiency and timing of gene rearrangement is controlled pharmacologically, a sparse subset of labeled cells can be generated from the set of CreER-expressing cells at any time during development. Histochemical visualization of alkaline phosphatase activity reveals neuronal morphology with strong and uniform labeling of all processes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1529-2401
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2314-22
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12657690-Alkaline Phosphatase,
pubmed-meshheading:12657690-Animals,
pubmed-meshheading:12657690-Brain,
pubmed-meshheading:12657690-Cell Lineage,
pubmed-meshheading:12657690-Clone Cells,
pubmed-meshheading:12657690-Gene Targeting,
pubmed-meshheading:12657690-Genes, Reporter,
pubmed-meshheading:12657690-Histocytochemistry,
pubmed-meshheading:12657690-Integrases,
pubmed-meshheading:12657690-Mice,
pubmed-meshheading:12657690-Neurons,
pubmed-meshheading:12657690-Phenotype,
pubmed-meshheading:12657690-Receptors, Estrogen,
pubmed-meshheading:12657690-Recombinant Fusion Proteins,
pubmed-meshheading:12657690-Recombination, Genetic,
pubmed-meshheading:12657690-Reproducibility of Results,
pubmed-meshheading:12657690-Selective Estrogen Receptor Modulators,
pubmed-meshheading:12657690-Tamoxifen,
pubmed-meshheading:12657690-Transgenes,
pubmed-meshheading:12657690-Viral Proteins
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pubmed:year |
2003
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pubmed:articleTitle |
A noninvasive genetic/pharmacologic strategy for visualizing cell morphology and clonal relationships in the mouse.
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pubmed:affiliation |
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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