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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
2003-6-2
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pubmed:abstractText |
PLU-1 is a large (1544 amino acids) nuclear protein that is highly expressed in breast cancers and is proposed to function as a regulator of gene expression. A yeast two-hybrid screen using PLU-1 as bait has identified two unrelated PLU-1 interacting proteins, namely brain factor-1 (BF-1) and paired box 9 (PAX9), both of which are developmental transcription factors. BF-1 and PAX9 interact with PLU-1 via a novel conserved sequence motif (Ala-X-Ala-Ala-X-Val-Pro-X4-Val-Pro-X8-Pro, termed the VP motif), because deletion or site-directed mutagenesis of this motif in either protein abolishes PLU-1 interaction in vivo. In a reporter assay system, PLU-1 has potent transcriptional repression activity. BF-1 and PAX9 also represses transcription in the same assay, but co-expression of PLU-1 with BF-1 or PAX9 significantly enhances this repression. Mutation of the PLU-1 binding motifs in BF-1 and PAX9 abolishes the observed PLU-1 co-repression activity. These data support a role for PLU-1 acting as a transcriptional co-repressor of two unrelated developmental transcription factors. Because both BF-1 and PAX proteins interact with members of the groucho co-repressor family, it is plausible that PLU-1 has a role in groucho-mediated transcriptional repression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Basic Helix-Loop-Helix...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Jumonji Domain-Containing Histone...,
http://linkedlifedata.com/resource/pubmed/chemical/KDM5B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PAX9 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/PAX9 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/groucho protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/polycomb group proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
20507-13
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12657635-Amino Acid Sequence,
pubmed-meshheading:12657635-Basic Helix-Loop-Helix Transcription Factors,
pubmed-meshheading:12657635-Binding Sites,
pubmed-meshheading:12657635-Cell Line,
pubmed-meshheading:12657635-Conserved Sequence,
pubmed-meshheading:12657635-DNA-Binding Proteins,
pubmed-meshheading:12657635-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:12657635-Humans,
pubmed-meshheading:12657635-Jumonji Domain-Containing Histone Demethylases,
pubmed-meshheading:12657635-Kidney,
pubmed-meshheading:12657635-Molecular Sequence Data,
pubmed-meshheading:12657635-Mutagenesis, Site-Directed,
pubmed-meshheading:12657635-Neoplasm Proteins,
pubmed-meshheading:12657635-Nerve Tissue Proteins,
pubmed-meshheading:12657635-Nuclear Proteins,
pubmed-meshheading:12657635-PAX9 Transcription Factor,
pubmed-meshheading:12657635-Repressor Proteins,
pubmed-meshheading:12657635-Transcription Factors,
pubmed-meshheading:12657635-Transcriptional Activation,
pubmed-meshheading:12657635-Two-Hybrid System Techniques
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pubmed:year |
2003
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pubmed:articleTitle |
Human PLU-1 Has transcriptional repression properties and interacts with the developmental transcription factors BF-1 and PAX9.
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pubmed:affiliation |
Centre for Structural Biology, Department of Biological Sciences, Imperial College London, Armstrong Road, London SW7 2AZ.
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pubmed:publicationType |
Journal Article
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