Linked Life Data

12653975

Source:http://linkedlifedata.com/resource/pubmed/id/12653975

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2003-3-25
pubmed:abstractText
Cell adhesion molecule-like receptor-type protein tyrosine phosphatases have been shown to be important for neurite outgrowth and neural development in several animal models. We have previously reported that in leucocyte common antigen-related (LAR) phosphatase deficient (LAR-deltaP) mice the number and size of basal forebrain cholinergic neurons, and their innervation of the hippocampal area, is reduced. In this study we compared the sprouting response of LAR-deficient and wildtype neurons in a peripheral and a central nervous system lesion model. Following sciatic nerve crush lesion, LAR-deltaP mice showed a delayed recovery of sensory, but not of motor, nerve function. In line with this, neurofilament-200 immunostaining revealed a significant reduction in the number of newly outgrowing nerve sprouts in LAR-deltaP animals. Morphometric analysis indicated decreased axonal areas in regenerating LAR-deltaP nerves when compared to wildtypes. Nonlesioned nerves in wildtype and LAR-deltaP mice did not differ regarding myelin and axon areas. Entorhinal cortex lesion resulted in collateral sprouting of septohippocampal cholinergic fibres into the dentate gyrus outer molecular layer in both genotype groups. However, LAR-deltaP mice demonstrated less increase in acetylcholinesterase density and fibre number at several time points following the lesion, indicating a delayed collateral sprouting response. Interestingly, a lesion-induced reduction in number of (septo-entorhinal) basal forebrain choline acetyltransferase-positive neurons occurred in both groups, whereas in LAR-deltaP mice the average cell body size was reduced as well. Thus, regenerative and collateral sprouting is significantly delayed in LAR-deficient mice, reflecting an important facilitative role for LAR in peripheral and central nervous system axonal outgrowth.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0953-816X
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
991-1005
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12653975-Acetylcholinesterase, pubmed-meshheading:12653975-Animals, pubmed-meshheading:12653975-Cell Count, pubmed-meshheading:12653975-Central Nervous System, pubmed-meshheading:12653975-Choline O-Acetyltransferase, pubmed-meshheading:12653975-Entorhinal Cortex, pubmed-meshheading:12653975-Immunohistochemistry, pubmed-meshheading:12653975-Male, pubmed-meshheading:12653975-Mice, pubmed-meshheading:12653975-Mice, Transgenic, pubmed-meshheading:12653975-Nerve Crush, pubmed-meshheading:12653975-Nerve Regeneration, pubmed-meshheading:12653975-Nerve Tissue Proteins, pubmed-meshheading:12653975-Neurofilament Proteins, pubmed-meshheading:12653975-Peripheral Nervous System, pubmed-meshheading:12653975-Protein Tyrosine Phosphatases, pubmed-meshheading:12653975-Receptor-Like Protein Tyrosine Phosphatases, Class 2, pubmed-meshheading:12653975-Receptors, Cell Surface, pubmed-meshheading:12653975-Recovery of Function, pubmed-meshheading:12653975-Sciatic Nerve
pubmed:year
2003
pubmed:articleTitle
Delayed peripheral nerve regeneration and central nervous system collateral sprouting in leucocyte common antigen-related protein tyrosine phosphatase-deficient mice.
pubmed:affiliation
Department of Cell Biology, Nijmegen Center for Molecular Life Sciences, UMC Radboud, University of Nijmegen, PO Box 9101, 6500 HB Nijmegen, The Netherlands. i.vanderzee@ncmls.kun.nl
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't