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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-3-31
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pubmed:abstractText |
New technologies are needed to characterize the migration, survival, and function of antigen-specific T cells in vivo. Here, we demonstrate that Epstein-Barr virus (EBV)--specific T cells transduced with vectors encoding herpes simplex virus-1 thymidine kinase (HSV-TK) selectively accumulate radiolabeled 2'-fluoro-2'-deoxy-1-beta-D-arabinofuranosyl-5-iodouracil (FIAU). After adoptive transfer, HSV-TK+ T cells labeled in vitro or in vivo with [131I]FIAU or [124I]FIAU can be noninvasively tracked in SCID mice bearing human tumor xenografts by serial images obtained by scintigraphy or positron emission tomography (PET), respectively. These T cells selectively accumulate in EBV+ tumors expressing the T cells' restricting HLA allele but not in EBV- or HLA-mismatched tumors. The concentrations of transduced T cells detected in tumors and tissues are closely correlated with the concentrations of label retained at each site. Radiolabeled transduced T cells retain their capacity to eliminate targeted tumors selectively. This technique for imaging the migration of ex vivo-transduced antigen-specific T cells in vivo is informative, nontoxic, and potentially applicable to humans.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1087-0156
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pubmed:author |
pubmed-author:BalatoniJuliusJ,
pubmed-author:BlasbergRonald GRG,
pubmed-author:BornmannWilliamW,
pubmed-author:DoubrovinMikhailM,
pubmed-author:DoubrovinaEkaterinaE,
pubmed-author:FinnRonaldR,
pubmed-author:GallardoHumilidad FHF,
pubmed-author:HellerGlennG,
pubmed-author:IvanovaAnnaA,
pubmed-author:KoehneGuentherG,
pubmed-author:LarsonSteven MSM,
pubmed-author:MayChadC,
pubmed-author:O'ReillyRichard JRJ,
pubmed-author:PonomarevVladimirV,
pubmed-author:RiviereIsabelleI,
pubmed-author:RuanShutianS,
pubmed-author:SadelainMichelM,
pubmed-author:Teruya-FeldsteinJulieJ,
pubmed-author:TjuvajevJuri G GelovaniJG,
pubmed-author:ZanzonicoPatP
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pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
405-13
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12652311-Animals,
pubmed-meshheading:12652311-Apoptosis,
pubmed-meshheading:12652311-Arabinofuranosyluracil,
pubmed-meshheading:12652311-Cell Line,
pubmed-meshheading:12652311-Cell Movement,
pubmed-meshheading:12652311-Cell Survival,
pubmed-meshheading:12652311-Herpesvirus 4, Human,
pubmed-meshheading:12652311-Humans,
pubmed-meshheading:12652311-Iodine Radioisotopes,
pubmed-meshheading:12652311-Isotope Labeling,
pubmed-meshheading:12652311-Mice,
pubmed-meshheading:12652311-Mice, SCID,
pubmed-meshheading:12652311-Neoplasm Transplantation,
pubmed-meshheading:12652311-Radiopharmaceuticals,
pubmed-meshheading:12652311-Sensitivity and Specificity,
pubmed-meshheading:12652311-Simplexvirus,
pubmed-meshheading:12652311-T-Lymphocytes,
pubmed-meshheading:12652311-Thymidine Kinase,
pubmed-meshheading:12652311-Tissue Distribution,
pubmed-meshheading:12652311-Tomography, Emission-Computed,
pubmed-meshheading:12652311-Transduction, Genetic,
pubmed-meshheading:12652311-Tumor Markers, Biological
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pubmed:year |
2003
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pubmed:articleTitle |
Serial in vivo imaging of the targeted migration of human HSV-TK-transduced antigen-specific lymphocytes.
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pubmed:affiliation |
Allogeneic Bone Marrow Transplantation Service, Department of Pediatrics, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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