Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-3-24
pubmed:abstractText
Initiation factor 3 (eIF3) forms a multifactor complex (MFC) with eIF1, eIF2, and eIF5 that stimulates Met-tRNA(i)(Met) binding to 40S ribosomes and promotes scanning or AUG recognition. We have previously characterized MFC subcomplexes produced in vivo from affinity-tagged eIF3 subunits lacking discrete binding domains for other MFC components. Here we asked whether these subcomplexes can bind to 40S ribosomes in vivo. We found that the N- and C-terminal domains of NIP1/eIF3c, the N- and C-terminal domains of TIF32/eIF3a, and eIF5 have critical functions in 40S binding, with eIF5 and the TIF32-CTD performing redundant functions. The TIF32-CTD interacted in vitro with helices 16-18 of domain I in 18S rRNA, and the TIF32-NTD and NIP1 interacted with 40S protein RPS0A. These results suggest that eIF3 binds to the solvent side of the 40S subunit in a way that provides access to the interface side for the two eIF3 segments (NIP1-NTD and TIF32-CTD) that interact with eIF1, eIF5, and the eIF2/GTP/Met-tRNA(i)(Met) ternary complex.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-10075937, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-10358023, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-11018020, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-11179233, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-11228145, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-11233977, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-11239155, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-11331597, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-11387228, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-11560931, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-11701127, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-11927566, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-12411506, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-12435632, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-1729602, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-1742346, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-2140872, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-3709551, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-7708014, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-7876188, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-9308967, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-9362495, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-9559554, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-9573242, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-9660829, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-9671501, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-9732867, http://linkedlifedata.com/resource/pubmed/commentcorrection/12651896-9826553
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0890-9369
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
786-99
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
The yeast eIF3 subunits TIF32/a, NIP1/c, and eIF5 make critical connections with the 40S ribosome in vivo.
pubmed:affiliation
Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development/NIH, Bethesda, MD 20892, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't