Linked Life Data

12650961

Source:http://linkedlifedata.com/resource/pubmed/id/12650961

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2003-3-24
pubmed:abstractText
The biochemical effects of permanent focal ischemia following unilateral occlusion of the middle cerebral artery in rats were studied by determining the content of specific proteins of the affected areas in the cerebral hemisphere. Brain proteins were prepared 72 h after the occlusion and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis. A significant increase in 66 and 80 kDa components and a paradoxical decrease in 260 kDa protein occurred in the ischemic brain tissues. The 66 and 80 kDa protein bands were identified as albumin and transferrin, respectively. The 260 kDa protein was analyzed by peptide mass fingerprinting (PMF) and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). The isoelectric point of the 260 kDa protein was 4.65 determined by isoelectric focusing. The data obtained from PMF were used in searching the protein database for homologous components. Three proteins with partial homology were identified. They were the microtubule-associated protein 1A, protein-tyrosine phosphatase zeta precursor (phosphacan), and protein kinase A anchoring protein 6. Polyclonal antibodies against the 260 kDa protein were raised and used to immunolocalize the antigen in various tissues. Positive staining occurred with brain neurons and pyramidal cells, islet cells, podocytes of kidney glomeruli, and endothelial cells of the venous sinuses of the spleen. The localization of 260 kDa protein strongly implies its function in these tissues. Its physiological and pathophysiological significances need to be clarified in future.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
967
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Identification of a key protein associated with cerebral ischemia.
pubmed:affiliation
Department of Pharmacology, School of Basic Medical Sciences, Peking University, Beijing 100083, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't