|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
22
|
|
pubmed:dateCreated |
2003-5-26
|
|
pubmed:abstractText |
Ski is a transcriptional co-repressor and is involved in the negative regulation of tumor growth factor-beta (TGF-beta) signaling. To understand more fully the role of Ski in TGF-beta signaling, we searched for novel Ski-interacting proteins. The identified C184M protein consists of 189 amino acids and contains the leucine-rich region. An association between Ski and C184M involving the leucine-rich region of C184M and the C-terminal coiled-coil motif of Ski was confirmed by glutathione S-transferase pull-down and immunoprecipitation assays. The C184M protein is located in the cytosol, and the C184M and Ski signals are co-localized in the cytoplasm when C184M was co-expressed with Ski in CV-1 cells. The cytoplasmic C184M-Ski complex inhibited the nuclear translocation of Smad2. Consistent with this, the activity of promoter containing the Smad-binding sites was repressed by C184M, and the TGF-beta-induced growth inhibition of mink lung Mv1Lu cells was attenuated by the ectopic expression of C184M. Thus, C184M inhibits TGF-beta signaling in concert with Ski. In hepatocytes, which express significant levels of C184M, the Ski signals were found only in the cytoplasm, supporting the notion that C184M forms a complex with Ski in the cytosol.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Receptor Coactivators,
http://linkedlifedata.com/resource/pubmed/chemical/SNW1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Skiip protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Smad Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
30
|
|
pubmed:volume |
278
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
20133-9
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:12646588-Animals,
pubmed-meshheading:12646588-Cell Line,
pubmed-meshheading:12646588-Cell Nucleus,
pubmed-meshheading:12646588-Cytoplasm,
pubmed-meshheading:12646588-DNA-Binding Proteins,
pubmed-meshheading:12646588-Humans,
pubmed-meshheading:12646588-Mice,
pubmed-meshheading:12646588-Mice, Inbred C57BL,
pubmed-meshheading:12646588-Nuclear Proteins,
pubmed-meshheading:12646588-Nuclear Receptor Coactivators,
pubmed-meshheading:12646588-Protein Binding,
pubmed-meshheading:12646588-Protein Transport,
pubmed-meshheading:12646588-Signal Transduction,
pubmed-meshheading:12646588-Smad Proteins,
pubmed-meshheading:12646588-Trans-Activators,
pubmed-meshheading:12646588-Transcription Factors,
pubmed-meshheading:12646588-Transforming Growth Factor beta,
pubmed-meshheading:12646588-Two-Hybrid System Techniques
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
The Ski-binding protein C184M negatively regulates tumor growth factor-beta signaling by sequestering the Smad proteins in the cytoplasm.
|
|
pubmed:affiliation |
Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Ibaraki 305-0074, Japan.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
