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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-3-20
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pubmed:abstractText |
VEGF is an important mediator of pathological angiogenesis in the eye and is a target for the development of novel anti-angiogenic molecules. In a previous study we identified 12-amino acid peptides derived from exon 6 of VEGF that inhibited VEGF binding to its receptors in HUVECs, endothelial cell functions, and in vitro angiogenesis. Screening of a series of truncated peptides corresponding to the inhibitory region of exon 6 identified a seven amino acid residue peptide, RKRKKSR, as the minimum exon 6-encoded sequence which retains the ability to inhibit VEGF receptor binding and angiogenesis in vitro. The effect of the seven-residue peptide was examined in a mouse model of ischaemic retinal neovascularisation. Administration of the peptide caused a 50% inhibition of retinal neovascularisation and was as effective in inhibiting ischaemic angiogenesis as soluble Flt-1 adenovirus. These results demonstrate that a seven amino acid VEGF exon 6-derived peptide is an effective inhibitor of ocular neovascularisation in vivo, and may have applications in the treatment of pathophysiological ocular neovascularisation in human disease.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
302
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
793-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12646239-Animals,
pubmed-meshheading:12646239-Cell Line,
pubmed-meshheading:12646239-Culture Media, Serum-Free,
pubmed-meshheading:12646239-Disease Models, Animal,
pubmed-meshheading:12646239-Endothelial Growth Factors,
pubmed-meshheading:12646239-Endothelium, Vascular,
pubmed-meshheading:12646239-Exons,
pubmed-meshheading:12646239-Female,
pubmed-meshheading:12646239-Humans,
pubmed-meshheading:12646239-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:12646239-Ischemia,
pubmed-meshheading:12646239-Lymphokines,
pubmed-meshheading:12646239-Mice,
pubmed-meshheading:12646239-Mice, Inbred C57BL,
pubmed-meshheading:12646239-Peptides,
pubmed-meshheading:12646239-Pregnancy,
pubmed-meshheading:12646239-Retina,
pubmed-meshheading:12646239-Retinal Neovascularization,
pubmed-meshheading:12646239-Vascular Endothelial Growth Factor A,
pubmed-meshheading:12646239-Vascular Endothelial Growth Factors
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pubmed:year |
2003
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pubmed:articleTitle |
A peptide encoded by exon 6 of VEGF (EG3306) inhibits VEGF-induced angiogenesis in vitro and ischaemic retinal neovascularisation in vivo.
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pubmed:affiliation |
Institute of Ophthalmology, Bath Street, London, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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