Linked Life Data

12646185

Source:http://linkedlifedata.com/resource/pubmed/id/12646185

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-3-20
pubmed:abstractText
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine expressed in the non-small lung carcinoma cells (NSCLC). However, transcriptional regulation of GM-CSF is not well characterized in NSCLC. In this study we found that two cis-acting ETS family consensus sites are important for transcriptional regulation of GM-CSF in A549 human lung carcinoma cells. These two sites are located separately at around -40 and -100 bp from the transcription start site. Results of transient transfection assays with A549 cells indicated that ETS2 had a strong positive effect on GM-CSF promoter activity. Furthermore, this activity was enhanced by protein kinase C activator, phorbol 12-myristate 13-acetate (PMA), in an ETS consensus-dependent manner, while PMA could also enhance the expression level of ETS2. The protein kinase C inhibitors decreased GM-CSF promoter activity induced by the protein kinase C activator PMA. We also found that antisense ETS2 mRNA decreased PMA-induced GM-CSF promoter activity, supporting the possibility that ETS2 is involved in protein kinase C-induced GM-CSF transcriptional function. Endogenous expression of GM-CSF mRNA was increased by ETS2 transfection and the increased expression was further enhanced by PMA. These data indicate that GM-CSF is up-regulated by ETS2, a target of protein kinase C.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
303
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
190-5
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12646185-Blotting, Western, pubmed-meshheading:12646185-Carcinoma, Non-Small-Cell Lung, pubmed-meshheading:12646185-DNA-Binding Proteins, pubmed-meshheading:12646185-Enzyme Activation, pubmed-meshheading:12646185-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:12646185-Humans, pubmed-meshheading:12646185-Lung Neoplasms, pubmed-meshheading:12646185-Plasmids, pubmed-meshheading:12646185-Promoter Regions, Genetic, pubmed-meshheading:12646185-Protein Binding, pubmed-meshheading:12646185-Protein Kinase C, pubmed-meshheading:12646185-Proto-Oncogene Protein c-ets-2, pubmed-meshheading:12646185-Proto-Oncogene Proteins, pubmed-meshheading:12646185-Repressor Proteins, pubmed-meshheading:12646185-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12646185-Trans-Activators, pubmed-meshheading:12646185-Transcription Factors, pubmed-meshheading:12646185-Transcriptional Activation, pubmed-meshheading:12646185-Transfection, pubmed-meshheading:12646185-Tumor Cells, Cultured, pubmed-meshheading:12646185-Up-Regulation
pubmed:year
2003
pubmed:articleTitle
ETS2 is involved in protein kinase C-activated expression of granulocyte-macrophage colony-stimulating factor in human non-small lung carcinoma cell line, A549.
pubmed:affiliation
Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't