Linked Life Data

12644664

Source:http://linkedlifedata.com/resource/pubmed/id/12644664

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-3-19
pubmed:abstractText
To understand further how pollination, seeds, auxin (4-chloroindole-3-acetic acid [4-Cl-IAA]), and gibberellins (GAs) regulate GA biosynthesis in pea (Pisum sativum) fruit, we studied expression of the gene PsGA3ox1 that codes for the enzyme that converts GA(20) to biologically active GA(1) using real-time reverse transcription-polymerase chain reaction analysis. PsGA3ox1 mRNA levels were minimally detectable in prepollinated pericarps and ovules (-2 d after anthesis [DAA]), increased dramatically after pollination (0 DAA), then decreased by 1 DAA. Seed PsGA3ox1 mRNA levels increased at 4 DAA and again 8 to 12 DAA, when seed development was rapid. Pericarp PsGA3ox1 mRNA levels peaked coincidentally with rapid pod diameter expansion (6-10 DAA) to accommodate the growing seeds. The effects of seeds and hormones on the expression of pericarp PsGA3ox1 were investigated over a 24-h treatment period. Pericarp PsGA3ox1 mRNA levels gradually increased from 2 to 3 DAA when seeds were present; however, when the seeds were removed, the pericarp transcript levels dramatically declined. When 2-DAA deseeded pericarps were treated with 4-Cl-IAA, PsGA3ox1 mRNA levels peaked 4 h after hormone treatment (270-fold increase), then decreased. PsGA3ox1 mRNA levels in deseeded pericarps treated with indole-3-acetic acid or GA(3) were the same or lower than deseeded controls. These data show that PsGA3ox1 is expressed and developmentally regulated in pea pericarps and seeds. These data also show that pericarp PsGA3ox1 expression is hormonally regulated and suggest that the conversion of GA(20) to GA(1) occurs in the pericarp and is regulated by the presence of seeds and 4-Cl-IAA for fruit growth.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12644664-10758505, http://linkedlifedata.com/resource/pubmed/commentcorrection/12644664-11013345, http://linkedlifedata.com/resource/pubmed/commentcorrection/12644664-11120474, http://linkedlifedata.com/resource/pubmed/commentcorrection/12644664-11950986, http://linkedlifedata.com/resource/pubmed/commentcorrection/12644664-12090620, http://linkedlifedata.com/resource/pubmed/commentcorrection/12644664-16653006, http://linkedlifedata.com/resource/pubmed/commentcorrection/12644664-16668557, http://linkedlifedata.com/resource/pubmed/commentcorrection/12644664-5673018, http://linkedlifedata.com/resource/pubmed/commentcorrection/12644664-8539289, http://linkedlifedata.com/resource/pubmed/commentcorrection/12644664-9238076, http://linkedlifedata.com/resource/pubmed/commentcorrection/12644664-9286112
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1137-46
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Pollination-, development-, and auxin-specific regulation of gibberellin 3beta-hydroxylase gene expression in pea fruit and seeds.
pubmed:affiliation
Plant Physiology and Molecular Biology Research Group, Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5. jocelyn.ozga@ualberta.ca
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't