Linked Life Data

12640028

Source:http://linkedlifedata.com/resource/pubmed/id/12640028

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 8
pubmed:dateCreated
2003-3-17
pubmed:abstractText
To explore mechanisms whereby Malpighian keratinocytes can transdifferentiate into an intestinal-like epithelium, as observed in the early steps of Barrett's esophagus (BE) development, long-standing cultures of esophageal keratinocytes derived from normal mouse esophageal explants were developed. These cells were able to form multilayers and to differentiate on filter support by the formation of differentiated layers of basal cells (cytokeratine 14 positive) on which secondary suprabasal cell layers (cytokeratine 4 positive) spontaneously developed. Thus, these cultured cells, referred to as P3E6, reproduced, at least in part, the proliferation and stratification pattern existing in the normal esophagus. Because chronic exposure to acid pH is known to be a critical factor for BE development, culture medium at pH 3.5 was added into the apical chamber of cell cultures. This led to a decrease in the overall number of cells but it did not affect cell proliferation. Furthermore, external acid environment triggered expression of the GFP reporter gene fused downstream of the cdx2 intestinal homeogene regulatory sequences in P3E6 transfected cells. Expression of the endogenous CDX2 protein, detected by western blot and immunocytochemical analysis, correlated with promoter activation. These findings demonstrate that chronic exposure of esophageal keratinocytes to acid pH induces transcription of cdx2, an intestinal specific homeobox gene known to play a critical role in the differentiation and maintenance of intestinal epithelial functions. The results suggest that chronic acid exposure can modify the fate of P3E6 esophageal keratinocytes towards an intestinal program. This can be a key step in the development of intestinal metaplasia often observed in esophagus-cardia junction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1429-36
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12640028-Adult, pubmed-meshheading:12640028-Animals, pubmed-meshheading:12640028-Barrett Esophagus, pubmed-meshheading:12640028-Caco-2 Cells, pubmed-meshheading:12640028-Cell Differentiation, pubmed-meshheading:12640028-Cell Division, pubmed-meshheading:12640028-Cells, Cultured, pubmed-meshheading:12640028-Epithelium, pubmed-meshheading:12640028-Esophagus, pubmed-meshheading:12640028-Gene Expression Regulation, pubmed-meshheading:12640028-Green Fluorescent Proteins, pubmed-meshheading:12640028-Homeodomain Proteins, pubmed-meshheading:12640028-Humans, pubmed-meshheading:12640028-Hydrochloric Acid, pubmed-meshheading:12640028-Hydrogen-Ion Concentration, pubmed-meshheading:12640028-Keratinocytes, pubmed-meshheading:12640028-Luminescent Proteins, pubmed-meshheading:12640028-Mice, pubmed-meshheading:12640028-Mice, Inbred BALB C, pubmed-meshheading:12640028-Microscopy, Confocal, pubmed-meshheading:12640028-Microscopy, Electron, pubmed-meshheading:12640028-Promoter Regions, Genetic, pubmed-meshheading:12640028-Recombinant Fusion Proteins, pubmed-meshheading:12640028-Time Factors, pubmed-meshheading:12640028-Trans-Activators
pubmed:year
2003
pubmed:articleTitle
Chronic acid exposure leads to activation of the cdx2 intestinal homeobox gene in a long-term culture of mouse esophageal keratinocytes.
pubmed:affiliation
Laboratory of Lympho-Epithelial Interactions, Department of Cell Biology and Infection, Pasteur Institute, 28, Rue du Dr Roux, 75015 Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't