Linked Life Data

12639216

Source:http://linkedlifedata.com/resource/pubmed/id/12639216

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-6-18
pubmed:abstractText
Oxidative stress plays a major pathogenetic role in cardiovascular disease. The C242T variant of the CYBA gene encoding the p22phox subunit of the NAD(P)H oxidase, a major source of superoxide production, has been shown to be associated with coronary artery disease and with vascular superoxide production in human veins ex vivo. Since superoxide degrades nitric oxide (NO), we hypothesized that the C242T variant influences endothelium-dependent vasodilation of the human forearm vasculature in vivo. In the present study, 90 subjects with elevated cholesterol levels were stratified for the C242T polymorphism of the CYBA p22phox gene. Endothelium-dependent and -independent vasodilation were assessed by plethysmographic monitoring of forearm blood flow responses to intra-arterial infusion of acetylcholine and sodium nitroprusside respectively. N(G)-Monomethyl-L-arginine (L-NMMA) was infused to analyse NO-mediated basal vascular tone. Baseline parameters (age, gender, blood pressure, body mass index, cholesterol level) were similar across the genotypes. No differences in forearm blood flow responses to the intra-arterial infusion of acetylcholine, sodium nitroprusside or L-NMMA were found across the CYBA p22phox genotypes. Our sample size of n =90 had a power of >80% (beta=0.20) with a P value of <0.05 (alpha=0.05) to detect a difference greater than 156% in the forearm blood flow response to acetylcholine across genotypes (S.D. 336%; average increase in forearm blood flow=514%). In conclusion, at a power of 80%, our study excludes a major effect of the C242T CYBA p22phox polymorphism on acetylcholine-mediated endothelium-dependent vasodilation and basal NO-mediated vascular tone of the human forearm circulation in subjects with hypercholesterolaemia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0143-5221
pubmed:author
pubmed:issnType
Print
pubmed:volume
105
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
97-103
pubmed:dateRevised
2006-5-4
pubmed:meshHeading
pubmed-meshheading:12639216-Acetylcholine, pubmed-meshheading:12639216-Adult, pubmed-meshheading:12639216-Analysis of Variance, pubmed-meshheading:12639216-Endothelium, Vascular, pubmed-meshheading:12639216-Female, pubmed-meshheading:12639216-Forearm, pubmed-meshheading:12639216-Genotype, pubmed-meshheading:12639216-Humans, pubmed-meshheading:12639216-Hypercholesterolemia, pubmed-meshheading:12639216-Male, pubmed-meshheading:12639216-Membrane Transport Proteins, pubmed-meshheading:12639216-Middle Aged, pubmed-meshheading:12639216-NADPH Dehydrogenase, pubmed-meshheading:12639216-NADPH Oxidase, pubmed-meshheading:12639216-Nitroprusside, pubmed-meshheading:12639216-Oxidative Stress, pubmed-meshheading:12639216-Phosphoproteins, pubmed-meshheading:12639216-Polymorphism, Genetic, pubmed-meshheading:12639216-Regional Blood Flow, pubmed-meshheading:12639216-Vasoconstrictor Agents, pubmed-meshheading:12639216-Vasodilation, pubmed-meshheading:12639216-Vasodilator Agents, pubmed-meshheading:12639216-omega-N-Methylarginine
pubmed:year
2003
pubmed:articleTitle
The C242T p22phox polymorphism and endothelium-dependent vasodilation in subjects with hypercholesterolaemia.
pubmed:affiliation
Universität Erlangen-Nürnberg, Medizinische Klinik IV/4, Klinikum Nürnberg Süd, Breslauer Strasse 201, D-90471 Nuremberg, Germany.
pubmed:publicationType
Journal Article