Linked Life Data

12634415

Source:http://linkedlifedata.com/resource/pubmed/id/12634415

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-3-13
pubmed:abstractText
Stem cell-based therapies for degenerative disorders and injuries are promising in the new era. Multipotential mesenchymal stem cells (MSCs) from bone marrow (BM) are on the leading edge because they are easy to expand in culture while maintaining their multilineage potential. In vitro assessment of the chondrogenic and osteogenic potentials of cultured MSCs has been established, and the BM used in those experiments was exclusively from healthy donors via iliac crest aspiration. It is unknown whether human marrow obtained from femurs also contains these multipotential MSCs. We collected marrow from proximal femurs of two patients undergoing total hip replacement surgery for femoral head osteonecrosis and isolated and culture expanded MSCs to about 20 population doublings. These cells were homogeneously positive for beta1-integrin. When pelleted into aggregates and cultured in a medium containing transforming growth factor-beta3 for 14 days, the cells began to express mRNA for aggrecan and collagen type II and to deposit immunoreactive collagen type II and sulfated proteoglycans in the matrix, hallmarks of chondrogenic differentiation. These MSCs could also be differentiated into osteocytic lineage in vitro, as shown by increased expression of alkaline phosphatase activity and deposition of mineral content onto culture plates. These results indicate that femoral BM obtained during hip surgeries also contained multipotential MSCs. These data imply that direct replacement therapy using MSCs from in situ marrow may be possible in the future and that an MSC bank may be established by using marrow from this approach, bypassing the necessity for iliac marrow aspiration from healthy donors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1066-5099
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
190-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12634415-Age Factors, pubmed-meshheading:12634415-Aged, pubmed-meshheading:12634415-Aggrecans, pubmed-meshheading:12634415-Bone Marrow Cells, pubmed-meshheading:12634415-Cell Differentiation, pubmed-meshheading:12634415-Cell Division, pubmed-meshheading:12634415-Cell Lineage, pubmed-meshheading:12634415-Cells, Cultured, pubmed-meshheading:12634415-Chondrocytes, pubmed-meshheading:12634415-Collagen Type I, pubmed-meshheading:12634415-Collagen Type II, pubmed-meshheading:12634415-Extracellular Matrix Proteins, pubmed-meshheading:12634415-Femur, pubmed-meshheading:12634415-Flow Cytometry, pubmed-meshheading:12634415-Humans, pubmed-meshheading:12634415-Lectins, C-Type, pubmed-meshheading:12634415-Male, pubmed-meshheading:12634415-Mesoderm, pubmed-meshheading:12634415-Middle Aged, pubmed-meshheading:12634415-Multipotent Stem Cells, pubmed-meshheading:12634415-Osteocytes, pubmed-meshheading:12634415-Osteonecrosis, pubmed-meshheading:12634415-Proteoglycans, pubmed-meshheading:12634415-RNA, Messenger, pubmed-meshheading:12634415-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12634415-Stem Cell Transplantation, pubmed-meshheading:12634415-Stromal Cells
pubmed:year
2003
pubmed:articleTitle
Multipotential mesenchymal stem cells from femoral bone marrow near the site of osteonecrosis.
pubmed:affiliation
Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University, College of Medicine, Taipei, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't