Linked Life Data

12634088

Source:http://linkedlifedata.com/resource/pubmed/id/12634088

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2003-3-13
pubmed:abstractText
Salmonella infections in reptiles, in contrast to those in birds and mammals, are limited to the intestinal tract. In this study, interactions of a strain of Salmonella enterica subsp. enterica serovar Muenchen (SEEM) with intestinal explants of the turtle Trachemys scripta scripta were examined by scanning electron microscopy (SEM). Adhesion and invasion in the chelonian intestinal explants at 30 degrees C and 37 degrees C were evaluated quantitatively. For purposes of comparison, the invasive capacity of SEEM in the continuous avian epithelial cell line DIV-1 at 30 degrees C and 37 degrees C was determined. Small numbers of M-like cells were found in the ileum of the turtles. The bacteria adhered mainly to the mucus of the intestinal explants. Only small numbers of salmonellae were associated with epithelial cells. Higher numbers of bacteria adhered at 30 degrees C than at 37 degrees C. Epithelial damage, embedding of bacteria in the epithelial surface and a ruffling-like process were noted only at 37 degrees C. Minimal numbers of salmonellae invaded the explants at 30 degrees C and 37 degrees C. Invasion of DIV-1 cells was greater at 37 degrees C than at 30 degrees C. The study suggested that the intestinal mucous layer provides an important site of colonization for salmonellae in the chelonian host and protects the underlying epithelial cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0021-9975
pubmed:author
pubmed:issnType
Print
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-26
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Interactions of Salmonella enterica subsp. enterica serovar Muenchen with intestinal explants of the turtle Trachemys scripta scripta.
pubmed:affiliation
Department of Pathology, Bacteriology and Avian Diseases, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't