Source:http://linkedlifedata.com/resource/pubmed/id/12630812
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2003-3-12
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| pubmed:abstractText |
Adrenomedullin (AM) is a multifunctional peptide involved in roles as varied as blood pressure regulation, growth, neurotransmission, and inflammation control, among others. We previously identified complement factor H as a serum binding protein for AM and showed that factor H regulates AM functions and vice versa. Here we searched for the specific binding sites for AM by using recombinant fragments of factor H and a non-radioactive binding assay with fluorescein-tagged AM. By this methodology, two specific binding sites for AM were found in factor H. One of them shows a high affinity for AM and is located at the carboxy terminal end of factor H, comprising short consensus repeats (SCR) 15-20. Smaller fragments of this region did not bind to AM efficiently, suggesting that the high affinity binding site of factor H requires a complex three-dimensional structure to recognize AM. Another binding site with lower affinity for AM was found in the middle of the factor H molecule, at SCR 8-11. Antibodies against factor H prevented AM binding altogether, but the main binding partner of factor H, C3b, did not, indicating that C3b and AM bind to different regions of factor H. These structure-function data support previous biochemical observations. Our understanding of the binding between AM and factor H may help in the development of new treatments for diseases in which these molecules play active roles.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0916-9636
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
26 Suppl
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
S55-9
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12630812-Adrenomedullin,
pubmed-meshheading:12630812-Binding Sites,
pubmed-meshheading:12630812-Complement Factor H,
pubmed-meshheading:12630812-Humans,
pubmed-meshheading:12630812-Peptides,
pubmed-meshheading:12630812-Protein Structure, Tertiary,
pubmed-meshheading:12630812-Structure-Activity Relationship
|
| pubmed:year |
2003
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| pubmed:articleTitle |
Mapping of the adrenomedullin-binding domains in human complement factor H.
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| pubmed:affiliation |
Cell and Cancer Biology Branch, Vascular Biology Faculty, National Cancer Institute, NIH, Building 10, Room 13N262, Bethesda, MD 20892, USA. martinea@mail.nih.gov
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| pubmed:publicationType |
Journal Article
|