Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-3-11
pubmed:abstractText
Corepressors N-CoR and SMRT participate in diverse repression pathways and exist in large protein complexes including HDAC3, TBL1 and TBLR1. However, the roles of these proteins in SMRT-N-CoR complex function are largely unknown. Here we report the purification and functional characterization of the human N-CoR complex. The purified N-CoR complex contains 10-12 associated proteins, including previously identified components and a novel actin-binding protein IR10. We show that TBL1/TBLR1 associates with N-CoR through two independent interactions: the N-terminal region and the C-terminal WD-40 repeats interact with the N-CoR RD1 and RD4 region, respectively. In vitro, TBL1/TBLR1 bind histones H2B and H4, and, importantly, repression by TBL1/TBLR1 correlates with their interaction with histones. By using specific small interference RNAs (siRNAs), we demonstrate that HDAC3 is essential, whereas TBL1 and TBLR1 are functionally redundant but essential for repression by unliganded thyroid hormone receptor. Together, our data reveal the roles of HDAC3 and TBL/TBLR1 and provide evidence for the functional importance of histone interaction in repression mediated by SMRT-N-CoR complexes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-10072350, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-10077563, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-10097068, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-10471500, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-10640275, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-10640276, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-10652267, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-10809664, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-10860984, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-10944117, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-11013263, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-11030619, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-11254656, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-11395403, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-11498575, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-11509652, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-11784848, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-11889044, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-11914274, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-11931768, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-12034878, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-12054897, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-1651714, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-2159385, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-2555064, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-6828386, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-7566114, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-7566127, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-7590246, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-8521507, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-8675011, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-8985118, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-9139820, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-9150135, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-9150137, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-9322436, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-9430643, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-9790534, http://linkedlifedata.com/resource/pubmed/commentcorrection/12628926-9885572
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Histone Deacetylases, http://linkedlifedata.com/resource/pubmed/chemical/Histones, http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments, http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thyroid Hormone, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins, http://linkedlifedata.com/resource/pubmed/chemical/TBL1X protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Transducin, http://linkedlifedata.com/resource/pubmed/chemical/coronin proteins, http://linkedlifedata.com/resource/pubmed/chemical/histone deacetylase 3
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1336-46
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
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