Linked Life Data

12627488

Source:http://linkedlifedata.com/resource/pubmed/id/12627488

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2003-3-10
pubmed:abstractText
Glycoproteins I and E of the Varicella-zoster virus, encoded by the neighbouring open reading frames 67 and 68, are transcribed into several transcript species that differ in size. From gI, three transcripts of 1.65, 2.7, and 3.6 kb are known, and from gE, two transcripts of 2.15 and 3.6 kb in size are known. Here, we demonstrate that these various transcript species appear in different amounts at different times post infection. At 12 hr post infection, the transcripts of 1.65 (gI) and 2.15 (gE) were clearly detectable, whereas the other transcripts appeared later on. RT-PCR studies using a set of different primers provided clear evidence that gI and gE are transcribed both, mono- and bicistronically, with predominance on the respective monocistronic transcript. Additional evidence for monocistronic transcription was found in the fact that both glycoproteins contain their own transcriptional start sites. Both promoter regions have their own basal transcription activity and include active TATA-boxes that were recognized by the TATA-box binding protein.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0146-6615
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
70 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
S51-5
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Analyses of the transcriptional pattern of glycoproteins E and I of Varicella-zoster virus and evidence for a monocistronic transcription.
pubmed:affiliation
Institute of Microbiology and Virology, University of Witten/Herdecke, Stockumer Strasse 10, D-58448 Witten, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't