Source:http://linkedlifedata.com/resource/pubmed/id/12626535
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2003-3-10
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| pubmed:abstractText |
Pre-TCR complexes are thought to signal in a ligand-independent manner because they are constitutively targeted to lipid rafts. We report that ligand-independent signaling is not a unique capability of the pre-TCR complex. Indeed, the TCR alpha subunit restores development of pT alpha-deficient thymocytes to the CD4(+)CD8(+) stage even in the absence of conventional MHC class I and class II ligands. Moreover, we found that pre-TCR and alpha beta TCR complexes exhibit no appreciable difference in their association with lipid rafts, suggesting that ligand-independence is a function of the CD4(-)CD8(-) (DN) thymocytes in which pre-TCR signaling occurs. In agreement, we found that only CD44(-)CD25(+) DN thymocytes (DN3) enabled activation of extracellular signal-regulated kinases by the pre-TCR complex. DN thymocytes also exhibited a lower signaling threshold relative to CD4(+)CD8(+) thymocytes, which was associated with both the markedly elevated lipid raft content of their plasma membranes and more robust capacitative Ca(2+) entry. Taken together these data suggest that cell-autonomous, ligand-independent signaling is primarily a property of the thymocytes in which pre-TCR signaling occurs.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/pre-T cell receptor alpha
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
|
| pubmed:volume |
170
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2853-61
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:12626535-Animals,
pubmed-meshheading:12626535-Cell Differentiation,
pubmed-meshheading:12626535-Enzyme Activation,
pubmed-meshheading:12626535-Ligands,
pubmed-meshheading:12626535-Lymphocyte Activation,
pubmed-meshheading:12626535-Membrane Glycoproteins,
pubmed-meshheading:12626535-Membrane Microdomains,
pubmed-meshheading:12626535-Mice,
pubmed-meshheading:12626535-Mice, Inbred C57BL,
pubmed-meshheading:12626535-Mice, Knockout,
pubmed-meshheading:12626535-Mice, SCID,
pubmed-meshheading:12626535-Mice, Transgenic,
pubmed-meshheading:12626535-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12626535-Organ Culture Techniques,
pubmed-meshheading:12626535-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:12626535-Signal Transduction,
pubmed-meshheading:12626535-Stem Cells,
pubmed-meshheading:12626535-T-Lymphocyte Subsets,
pubmed-meshheading:12626535-Thymus Gland
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| pubmed:year |
2003
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| pubmed:articleTitle |
Low activation threshold as a mechanism for ligand-independent signaling in pre-T cells.
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| pubmed:affiliation |
Division of Basic Sciences, Immunobiology Working Group, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|