Linked Life Data

12623075

Source:http://linkedlifedata.com/resource/pubmed/id/12623075

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-3-7
pubmed:abstractText
Mg(2+) competitively inhibits spermine transport in energized rat liver mitochondria (RLM) and exhibits a K(i) of 0.1mM on the initial rate and an I(50) of 0.6mM on total spermine accumulation after 20 min. Addition of 2mM Mg(2+) after spermine accumulation induces release of the polyamine. In view of the fact that spermine cycles across the inner membrane under physiological conditions, these results demonstrate that Mg(2+) inhibits spermine influx but does not affect the efflux pathway of the polyamine; the inhibitory effect occurs via an interaction with the specific site responsible for spermine transport. Instead, spermine inhibits Mg(2+) binding without affecting the rate of Mg(2+) transport, suggesting that both cations bind to the same site, which, however, is not used for Mg(2+) transport. Spermine also inhibits Mg(2+) efflux from RLM induced under conditions of the "low conductance state," a preliminary step preceding permeability transition pore opening.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0003-9861
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
411
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
262-6
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Reciprocal effects between spermine and Mg2+ on their movements across the mitochondrial membrane.
pubmed:affiliation
Dipartimento di Chimica Biologica, Istituto di Neuroscienze del C.N.R., Universita' di Padova, Unita' per lo Studio delle Biomembrane, Via G. Colombo 3, 35121 Padova, Italy.
pubmed:publicationType
Journal Article