Source:http://linkedlifedata.com/resource/pubmed/id/12622816
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2003-3-7
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pubmed:abstractText |
PrfA is the major regulator of Listeria virulence gene expression. This protein is a member of the Crp/Fnr family of transcription regulators. To gain a deeper understanding of the PrfA regulon, we constructed a whole-genome array based on the complete genome sequence of Listeria monocytogenes strain EGDe and evaluated the expression profiles of the wild-type EGDe and a prfA-deleted mutant (EGDe Delta prfA). Both strains were grown at 37 degrees C in brain-heart infusion broth (BHI) and BHI supplemented with either activated charcoal, a compound known to enhance virulence gene expression, or cellobiose, a sugar reported to downregulate virulence gene expression in spite of full expression of PrfA. We identified three groups of genes that are regulated differently. Group I comprises, in addition to the 10 already known genes, two new genes, lmo2219 and lmo0788, both positively regulated and preceded by a putative PrfA box. Group II comprises eight negatively regulated genes: lmo0278 is preceded by a putative PrfA box, and the remaining seven genes (lmo0178-lmo0184) are organized in an operon. Group III comprises 53 genes, of which only two (lmo0596 and lmo2067) are preceded by a putative PrfA box. Charcoal addition induced upregulation of group I genes but abolished regulation by PrfA of most group III genes. In the presence of cellobiose, all the group I genes were downregulated, whereas group III genes remained fully activated. Group II genes were repressed in all conditions tested. A comparison of the expression profiles between a second L. monocytogenes strain (P14), its spontaneous mutant expressing a constitutively active PrfA variant (P14prfA*) and its corresponding prfA-deleted mutant (P14 Delta prfA) and the EGDe strain revealed interesting strain-specific differences. Sequences strongly similar to a sigma B-dependent promoter were identified upstream of 22 group III genes. These results suggest that PrfA positively regulates a core set of 12 genes preceded by a PrfA box and probably expressed from a sigma A-dependent promoter. In contrast, a second set of PrfA-regulated genes lack a PrfA box and are expressed from a sigma B-dependent promoter. This study reveals that PrfA can act as an activator or a repressor and suggests that PrfA may directly or indirectly activate different sets of genes in association with different sigma factors.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cellobiose,
http://linkedlifedata.com/resource/pubmed/chemical/Charcoal,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Termination Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0950-382X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1613-25
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12622816-Bacterial Proteins,
pubmed-meshheading:12622816-Cell Division,
pubmed-meshheading:12622816-Cellobiose,
pubmed-meshheading:12622816-Charcoal,
pubmed-meshheading:12622816-Culture Media,
pubmed-meshheading:12622816-Gene Expression Profiling,
pubmed-meshheading:12622816-Gene Expression Regulation, Bacterial,
pubmed-meshheading:12622816-Genes, Bacterial,
pubmed-meshheading:12622816-Listeria monocytogenes,
pubmed-meshheading:12622816-Mutation,
pubmed-meshheading:12622816-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:12622816-Peptide Termination Factors,
pubmed-meshheading:12622816-Polymerase Chain Reaction,
pubmed-meshheading:12622816-Trans-Activators,
pubmed-meshheading:12622816-Transcription, Genetic
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pubmed:year |
2003
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pubmed:articleTitle |
Transcriptome analysis of Listeria monocytogenes identifies three groups of genes differently regulated by PrfA.
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pubmed:affiliation |
Unité des Interactions Bactéries-Cellules and Laboratoire de Génomique des Microorganismes Pathogènes, Institut Pasteur, 28, Rue du Docteur Roux, 75724 Paris Cedex 15, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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