Linked Life Data

12620841

Source:http://linkedlifedata.com/resource/pubmed/id/12620841

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-3-6
pubmed:abstractText
The role of hydrogenase on the reduction of 2,4,6-trinitrotoluene (TNT) in Clostridium acetobutylicum was evaluated. An Fe-only hydrogenase was isolated and identified by using TNT reduction activity as the selection basis. The formation of hydroxylamino intermediates by the purified enzyme corresponded to expected products for this reaction, and saturation kinetics were determined with a K(m) of 152 micro M. Comparisons between the wild type and a mutant strain lacking the region encoding an alternative Fe-Ni hydrogenase determined that Fe-Ni hydrogenase activity did not significantly contribute to TNT reduction. Hydrogenase expression levels were altered in various strains, allowing study of the role of the enzyme in TNT reduction rates. The level of hydrogenase activity in a cell system correlated (R(2) = 0.89) with the organism's ability to reduce TNT. A strain that overexpressed the hydrogenase activity resulted in maintained TNT reduction during late growth phases, which it is not typically observed in wild type strains. Strains exhibiting underexpression of hydrogenase produced slower TNT rates of reduction correlating with the determined level of expression. The isolated Fe-only hydrogenase is the primary catalyst for reducing TNT nitro substituents to the corresponding hydroxylamines in C. acetobutylicum in whole-cell systems. A mechanism for the reaction is proposed. Due to the prevalence of hydrogenase in soil microbes, this research may enhance the understanding of nitroaromatic compound transformation by common microbial communities.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-10049845, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-10529166, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-10742229, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-11160107, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-11466286, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-1368230, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-1673680, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-2268159, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-779650, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-8386500, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-8626337, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-8760920, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-9007006, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-9286999, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-9402066, http://linkedlifedata.com/resource/pubmed/commentcorrection/12620841-9930693
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1542-7
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
2,4,6-trinitrotoluene reduction by an Fe-only hydrogenase in Clostridium acetobutylicum.
pubmed:affiliation
Civil and Environmental Engineering, Rice University, Houston, TX 77005-1892, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't