Source:http://linkedlifedata.com/resource/pubmed/id/12620809
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2003-3-6
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| pubmed:abstractText |
We have constructed a series of plasmids encoding premembrane (prM) and envelope (E) protein genes of dengue virus type 2 (DEN-2). These plasmids included an authentic DEN-2 prM-E construct (pCBD2-14-6), and two chimeric constructs, 90% DEN-2 E-10% Japanese encephalitis (JE) virus E (pCB9D2-1J-4-3) and 80% DEN-2 E-20% JE E (pCB8D2-2J-2-9-1). Monoclonal antibody (MAb) reactivity indicated that all three plasmids expressed authentic DEN-2 virus E protein epitopes representative of flavivirus domains 1, 2, and 3. However, only the pCB8D2-2J-2-9-1 construct secreted high levels of prM, M (membrane), and E proteins into the culture fluid of plasmid-transformed COS-1 cells. The major portion of the prM and E proteins expressed by COS-1 cells transformed by pCBD2-14-6 or pCB9D2-4-3 plasmids remained membrane-bound. The results supported the notion that an unidentified membrane retention sequence is located between E-397 and E-436 of DEN-2 virus E protein. Replacing the carboxyl-terminal 20% of DEN-2 E (397-450) with the corresponding JE sequence had no effect on anti-DEN-2 MAb reactivity, indicating that this region is antigenically inert, although it is required for antigen secretion. Plasmid pCBD2-2J-2-9-1, which expressed secreted forms of prM/M and E that have the potential to form subviral particles, was superior to other constructs in stimulating an antibody response. Ninety percent neutralization titers ranging from 1:40 to >1:1000 were observed in seven of nine serum specimens from pCB8D2-2J-2-9-1-immunized mice. Eleven of twelve 2-day-old neonatal mice, derived from a pCB8D2-2J-2-9-1 immunized female mouse, survived intraperitoneal challenge of DEN-2 New Guinea C virus.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/E-glycoprotein, Dengue virus type 2,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/prM protein, Flavivirus
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0042-6822
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
306
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
170-80
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| pubmed:dateRevised |
2006-5-1
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| pubmed:meshHeading |
pubmed-meshheading:12620809-Animals,
pubmed-meshheading:12620809-Antibodies, Viral,
pubmed-meshheading:12620809-COS Cells,
pubmed-meshheading:12620809-Cell Membrane,
pubmed-meshheading:12620809-Cercopithecus aethiops,
pubmed-meshheading:12620809-Dengue,
pubmed-meshheading:12620809-Dengue Virus,
pubmed-meshheading:12620809-Encephalitis Virus, Japanese,
pubmed-meshheading:12620809-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12620809-Epitope Mapping,
pubmed-meshheading:12620809-Female,
pubmed-meshheading:12620809-Male,
pubmed-meshheading:12620809-Mice,
pubmed-meshheading:12620809-Mice, Inbred ICR,
pubmed-meshheading:12620809-Neutralization Tests,
pubmed-meshheading:12620809-Plasmids,
pubmed-meshheading:12620809-Recombinant Fusion Proteins,
pubmed-meshheading:12620809-Vaccination,
pubmed-meshheading:12620809-Vero Cells,
pubmed-meshheading:12620809-Viral Envelope Proteins,
pubmed-meshheading:12620809-Viral Vaccines
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| pubmed:year |
2003
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| pubmed:articleTitle |
Enhancing biosynthesis and secretion of premembrane and envelope proteins by the chimeric plasmid of dengue virus type 2 and Japanese encephalitis virus.
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| pubmed:affiliation |
Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Service, Post Office Box 2087, Fort Collins, CO 80522, USA. gxc7@cdc.gov
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| pubmed:publicationType |
Journal Article
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