Source:http://linkedlifedata.com/resource/pubmed/id/12619801
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2003-3-6
|
| pubmed:databankReference | |
| pubmed:abstractText |
Using two-dimensional (2D)-PAGE, partial protein internal sequencing, and PCR with degenerate primers, we cloned a novel cDNA named HEP21 from hen egg white. The 0.5-kb cDNA encodes a 106 amino acid protein with a cysteine spacing pattern suggesting that HEP21 is a new member of the uPAR/CD59/Ly-6/ snake neurotoxin superfamily. The closest homology of HEP21 is to mouse Ly-6C. Unlike most members of this protein family, HEP21 is not glycosylphosphatidylinositol (GPI)-anchored but is a secreted protein, as indicated by its localization and the presence of a signal peptide in its sequence. Moreover, HEP21 appears as an original member of this protein superfamily because it is predominantly expressed in a tissue, i.e., the oviduct, and especially the magnum where the egg white components are secreted.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0032-5791
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
82
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
242-50
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12619801-Amino Acid Sequence,
pubmed-meshheading:12619801-Animals,
pubmed-meshheading:12619801-Base Sequence,
pubmed-meshheading:12619801-Chickens,
pubmed-meshheading:12619801-Cloning, Molecular,
pubmed-meshheading:12619801-DNA, Complementary,
pubmed-meshheading:12619801-Egg Proteins,
pubmed-meshheading:12619801-Egg White,
pubmed-meshheading:12619801-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:12619801-Female,
pubmed-meshheading:12619801-Gene Expression,
pubmed-meshheading:12619801-Molecular Sequence Data,
pubmed-meshheading:12619801-Oviducts,
pubmed-meshheading:12619801-Random Amplified Polymorphic DNA Technique,
pubmed-meshheading:12619801-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12619801-Sequence Analysis, Protein,
pubmed-meshheading:12619801-Sequence Homology
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Cloning and characterization of HEP21, a new member of the uPAR/Ly6 protein superfamily predominantly expressed in hen egg white.
|
| pubmed:affiliation |
UMR INRA-ENSAR 1055 Physico-chimie et Technologie des Ovoproduits, Pole d'Enseignement Supérieur et de Recherche Agronomique de Rennes, 65 rue de Saint-Brieuc CS 84215-35042 Rennes cedex, France. nau@agrorennes.educagri.fr
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|