Linked Life Data

12615971

Source:http://linkedlifedata.com/resource/pubmed/id/12615971

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 7
pubmed:dateCreated
2003-3-4
pubmed:abstractText
Protistan cells employ a wide variety of strategies to reinforce and give pattern to their outermost cortical layers. Whereas some use common cytoskeletal elements such as microtubules, others are based on novel cytoskeletal proteins that are as-yet-unknown in higher eukaryotes. The hypotrich ciliate Euplotes possesses a continuous monolayer of scales or plates, located within flattened membranous sacs ('alveoli') just below the plasma membrane, and this provides rigidity and form to the cell. Using immunological techniques, the major proteins comprising these 'alveolar plates' have been identified and termed alpha-, beta-, and gamma-plateins. The present report describes work leading to the molecular characterization of three plateins, alpha 1 and alpha 2 (predicted M(r)s of 61 and 56 kDa) and a beta/gamma form (M(r)=73 kDa). All three proteins have features that are hallmarks of articulins, a class of cytoskeletal proteins that has been identified in the cortex of a wide variety of protistan cells, including certain flagellates, ciliates, dinoflagellates and PLASMODIUM: Chief among these common features are a prominent primary domain of tandem 12-amino acid repeats, rich in valine and proline, and a secondary domain of fewer, shorter repeating units. However, variations in amino acid use within both primary and secondary repetitive domains, and a much more acidic character (predicted pIs of 4.7-4.9), indicate that the plateins represent the first proteins in a new subclass or family of articulins. This conclusion is supported by another novel feature of the plateins, the presence of a canonical hydrophobic signal peptide at the N-terminus of each derived platein sequence. This correlates well with the final cellular location of the plateins, which are assembled into plates within the membrane-limited alveolar sacs. To our knowledge, this is the first report in any eukaryote of cytoskeletal proteins with such start-transfer sequences. Confocal immunofluorescence microscopy, using antibodies to the plateins as probes, reveals that new alveolar plates (enlarging in cortical zones undergoing morphogenesis) label more faintly than mature parental plates. During plate assembly (or polymerization), the plateins thus appear to exist in a more soluble form.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1291-303
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12615971-Animals, pubmed-meshheading:12615971-Cell Compartmentation, pubmed-meshheading:12615971-Cell Membrane, pubmed-meshheading:12615971-Cell Size, pubmed-meshheading:12615971-Cytoskeletal Proteins, pubmed-meshheading:12615971-Cytoskeleton, pubmed-meshheading:12615971-DNA, Complementary, pubmed-meshheading:12615971-Euplotes, pubmed-meshheading:12615971-Evolution, Molecular, pubmed-meshheading:12615971-Fluorescent Antibody Technique, pubmed-meshheading:12615971-Membrane Proteins, pubmed-meshheading:12615971-Molecular Sequence Data, pubmed-meshheading:12615971-Phylogeny, pubmed-meshheading:12615971-Protein Sorting Signals, pubmed-meshheading:12615971-Protein Structure, Tertiary, pubmed-meshheading:12615971-Protozoan Proteins, pubmed-meshheading:12615971-Sequence Homology, Amino Acid, pubmed-meshheading:12615971-Sequence Homology, Nucleic Acid
pubmed:year
2003
pubmed:articleTitle
Cytoskeletal proteins with N-terminal signal peptides: plateins in the ciliate Euplotes define a new family of articulins.
pubmed:affiliation
Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21250, USA. kloetzel@umbc.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't