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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5611
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pubmed:dateCreated |
2003-2-28
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pubmed:abstractText |
The mechanisms that determine how folding attempts are interrupted to target folding-incompetent proteins for endoplasmic reticulum-associated degradation (ERAD) are poorly defined. Here the alpha-mannosidase I-like protein EDEM was shown to extract misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. EDEM overexpression resulted in faster release of folding-incompetent proteins from the calnexin cycle and earlier onset of degradation, whereas EDEM down-regulation prolonged folding attempts and delayed ERAD. Up-regulation of EDEM during ER stress may promote cell recovery by clearing the calnexin cycle and by accelerating ERAD of terminally misfolded polypeptides.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1095-9203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
28
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pubmed:volume |
299
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1397-400
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12610306-Aspartic Acid Endopeptidases,
pubmed-meshheading:12610306-Calnexin,
pubmed-meshheading:12610306-Cell Line,
pubmed-meshheading:12610306-Down-Regulation,
pubmed-meshheading:12610306-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12610306-Endoplasmic Reticulum,
pubmed-meshheading:12610306-Glycoproteins,
pubmed-meshheading:12610306-Glycosylation,
pubmed-meshheading:12610306-Humans,
pubmed-meshheading:12610306-Kinetics,
pubmed-meshheading:12610306-Membrane Proteins,
pubmed-meshheading:12610306-Molecular Weight,
pubmed-meshheading:12610306-Polysaccharides,
pubmed-meshheading:12610306-Protein Conformation,
pubmed-meshheading:12610306-Protein Folding,
pubmed-meshheading:12610306-RNA Interference,
pubmed-meshheading:12610306-Transfection,
pubmed-meshheading:12610306-Up-Regulation
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pubmed:year |
2003
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pubmed:articleTitle |
Role of EDEM in the release of misfolded glycoproteins from the calnexin cycle.
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pubmed:affiliation |
Institute for Research in Biomedicine, CH-6500 Bellinzona, Switzerland. Maurizio.molinari@irb.unisi.ch
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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