Linked Life Data

12609047

Source:http://linkedlifedata.com/resource/pubmed/id/12609047

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-2-28
pubmed:databankReference
pubmed:abstractText
The transcription factors DREBs/CBFs specifically interact with the dehydration-responsive element/C-repeat (DRE/CRT) cis-acting element (core motif: G/ACCGAC) and control the expression of many stress-inducible genes in Arabidopsis. In rice, we isolated five cDNAs for DREB homologs: OsDREB1A, OsDREB1B, OsDREB1C, OsDREB1D, and OsDREB2A. Expression of OsDREB1A and OsDREB1B was induced by cold, whereas expression of OsDREB2A was induced by dehydration and high-salt stresses. The OsDREB1A and OsDREB2A proteins specifically bound to DRE and activated the transcription of the GUS reporter gene driven by DRE in rice protoplasts. Over-expression of OsDREB1A in transgenic Arabidopsis induced over-expression of target stress-inducible genes of Arabidopsis DREB1A resulting in plants with higher tolerance to drought, high-salt, and freezing stresses. This indicated that OsDREB1A has functional similarity to DREB1A. However, in microarray and RNA blot analyses, some stress-inducible target genes of the DREB1A proteins that have only ACCGAC as DRE were not over-expressed in the OsDREB1A transgenic Arabidopsis. The OsDREB1A protein bound to GCCGAC more preferentially than to ACCGAC whereas the DREB1A proteins bound to both GCCGAC and ACCGAC efficiently. The structures of DREB1-type ERF/AP2 domains in monocots are closely related to each other as compared with that in the dicots. OsDREB1A is potentially useful for producing transgenic monocots that are tolerant to drought, high-salt, and/or cold stresses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0960-7412
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
751-63
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12609047-Adaptation, Physiological, pubmed-meshheading:12609047-Amino Acid Sequence, pubmed-meshheading:12609047-Arabidopsis, pubmed-meshheading:12609047-Base Sequence, pubmed-meshheading:12609047-Binding Sites, pubmed-meshheading:12609047-Cold Temperature, pubmed-meshheading:12609047-DNA-Binding Proteins, pubmed-meshheading:12609047-Disasters, pubmed-meshheading:12609047-Electrophoretic Mobility Shift Assay, pubmed-meshheading:12609047-Gene Expression Regulation, Plant, pubmed-meshheading:12609047-Glucuronidase, pubmed-meshheading:12609047-Molecular Sequence Data, pubmed-meshheading:12609047-Oryza sativa, pubmed-meshheading:12609047-Phylogeny, pubmed-meshheading:12609047-Plant Proteins, pubmed-meshheading:12609047-Plants, Genetically Modified, pubmed-meshheading:12609047-Recombinant Fusion Proteins, pubmed-meshheading:12609047-Sequence Homology, Amino Acid, pubmed-meshheading:12609047-Sodium Chloride, pubmed-meshheading:12609047-Trans-Activators, pubmed-meshheading:12609047-Water
pubmed:year
2003
pubmed:articleTitle
OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression.
pubmed:affiliation
Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't