Source:http://linkedlifedata.com/resource/pubmed/id/12606475
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2003-4-22
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| pubmed:abstractText |
Long-term preservation of mouse sperm by desiccation is economically and logistically attractive. The current investigation is a feasibility study of the preservation of mouse sperm by convective drying in an inert gas (nitrogen). Mouse sperm from the B6D2F1 strain isolated in an EGTA-supplemented Tris-HCl buffer were dried using three different drying rates and were stored for 18-24 h at 4 degrees C. The mean final moisture content was <5% for all the protocols. After intracytoplasmic sperm injection (ICSI), the mean blastocyst formation rates were 64%, 58%, and 35% using the rapid-, moderate-, and slow-drying protocols, respectively. The slow-drying protocol resulted in a rate of development significantly lower than that observed using rapid- and moderate-drying protocols and indicated that a slower drying rate may be detrimental to the DNA integrity of mouse sperm. The transfer of 85 two- or four-cell embryos that were produced using rapidly desiccated sperm resulted in 11 fetuses (13%) on Day 15 compared with the production of 34 fetuses (40%) produced using the transfer of 86 two- or four-cell embryos that were produced using fresh sperm (P < 0.05). The results demonstrate the feasibility of using a convective drying protocol for the successful desiccation of mouse sperm and identifies some of the important parameters required for optimization of the procedure.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0006-3363
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
68
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1779-86
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:12606475-Animals,
pubmed-meshheading:12606475-Blastocyst,
pubmed-meshheading:12606475-Buffers,
pubmed-meshheading:12606475-Convection,
pubmed-meshheading:12606475-Culture Media,
pubmed-meshheading:12606475-Desiccation,
pubmed-meshheading:12606475-Edetic Acid,
pubmed-meshheading:12606475-Embryo Implantation,
pubmed-meshheading:12606475-Embryonic and Fetal Development,
pubmed-meshheading:12606475-Female,
pubmed-meshheading:12606475-Fluid Therapy,
pubmed-meshheading:12606475-Freeze Drying,
pubmed-meshheading:12606475-Insemination, Artificial,
pubmed-meshheading:12606475-Male,
pubmed-meshheading:12606475-Mice,
pubmed-meshheading:12606475-Noble Gases,
pubmed-meshheading:12606475-Oocytes,
pubmed-meshheading:12606475-Ovum,
pubmed-meshheading:12606475-Pregnancy,
pubmed-meshheading:12606475-Spermatozoa,
pubmed-meshheading:12606475-Temperature
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Desiccation tolerance of spermatozoa dried at ambient temperature: production of fetal mice.
|
| pubmed:affiliation |
Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston 02115, USA.
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| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
|