Source:http://linkedlifedata.com/resource/pubmed/id/12604647
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2003-2-26
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| pubmed:abstractText |
Effects of several Cl(-) channel blockers on ionic currents in mouse embryos were studied using whole-cell patch-clamp and microelectrode methods. Microelectrode measurements showed that the resting membrane potential of early embryonic cells (1-cell stage) was -23 mV and that reduction of extracellular Cl(-) concentration depolarized the membrane, suggesting that Cl(-) conductance is a major contributor for establishing the resting membrane potential. Membrane currents recorded by whole-cell voltage clamp showed outward rectification and confirmed that a major component of these embryonic currents are carried by Cl(-) ions. A Cl(-) channel blocker, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), suppressed the outward rectifier current in a voltage- and concentration-dependent manner. Other Cl(-) channel blockers (5-nitro-2-[3-phenylpropyl-amino] benzoic acid and 2-[3-(trifluoromethyl)-anilino] nicotinic acid [niflumic acid]) similarly inhibited this current. Simultaneous application of niflumic acid with DIDS further suppressed the outward rectifier current. Under high osmotic condition, niflumic acid, but not DIDS, inhibited the Cl(-)current, suggesting the presence of two types of Cl(-) channels: a DIDS-sensitive (swelling-activated) channel, and a DIDS-insensitive (niflumic acid-sensitive) Cl(-) channel. Anion permeability of the DIDS-insensitive Cl(-) current differed from that of the compound Cl(-) current: Rank order of anion permeability of the DIDS-sensitive Cl(-) channels was I(-) = Br(-) > Cl(-) > gluconate(-), whereas that of the DIDS-insensitive Cl(-) channel was I(-) = Br(-) > Cl(-) >> gluconate(-). These results indicate that early mouse embryos have a Cl(-) channel that is highly permeable to amino acids, which may regulate intracellular amino acid concentration.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4,4'-Diisothiocyanostilbene-2,2'-Dis...,
http://linkedlifedata.com/resource/pubmed/chemical/5-nitro-2-(3-phenylpropylamino)benzo...,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Chloride Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Gluconates,
http://linkedlifedata.com/resource/pubmed/chemical/Niflumic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrobenzoates,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/gluconic acid
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0006-3363
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
68
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
947-53
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12604647-4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid,
pubmed-meshheading:12604647-Amino Acids,
pubmed-meshheading:12604647-Animals,
pubmed-meshheading:12604647-Chloride Channels,
pubmed-meshheading:12604647-Embryo, Mammalian,
pubmed-meshheading:12604647-Female,
pubmed-meshheading:12604647-Gluconates,
pubmed-meshheading:12604647-Male,
pubmed-meshheading:12604647-Membrane Potentials,
pubmed-meshheading:12604647-Mice,
pubmed-meshheading:12604647-Mice, Inbred C3H,
pubmed-meshheading:12604647-Mice, Inbred C57BL,
pubmed-meshheading:12604647-Mice, Inbred ICR,
pubmed-meshheading:12604647-Niflumic Acid,
pubmed-meshheading:12604647-Nitrobenzoates,
pubmed-meshheading:12604647-Osmolar Concentration,
pubmed-meshheading:12604647-Patch-Clamp Techniques,
pubmed-meshheading:12604647-Potassium,
pubmed-meshheading:12604647-Pregnancy,
pubmed-meshheading:12604647-RNA, Messenger,
pubmed-meshheading:12604647-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12604647-Sodium
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| pubmed:year |
2003
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| pubmed:articleTitle |
Amino acid-permeable anion channels in early mouse embryos and their possible effects on cleavage.
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| pubmed:affiliation |
Department of Obstetrics and Gynecology, School of Medicine, Fukuoka University, Fukuoka 814-0180, Japan. momo-s@cis.fukuoka-u.ac.jp
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| pubmed:publicationType |
Journal Article
|