Linked Life Data

12602868

Source:http://linkedlifedata.com/resource/pubmed/id/12602868

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-2-26
pubmed:databankReference
pubmed:abstractText
The Arabidopsis genome possesses a number of sequences that are predicted to encode proteins that are similar to mammalian and yeast polyadenylation factor subunits. One of these resides on chromosome V and has the potential to encode a polypeptide related to the 100 kDa subunit of the mammalian cleavage and polyadenylation specificity factor (CPSF). This gene encodes a ca. 2400 nucleotide mRNA that in turn can be translated to yield a polypeptide that is 39% identical to the mammalian CPSF100 protein. Antibodies raised against the Arabidopsis protein recognized distinctive polypeptides in nuclear extracts prepared from pea and wheat germ, consistent with the hypothesis that the Arabidopsis protein is resident in a nuclear polyadenylation complex. Interestingly, the Arabidopsis CPSF100 was found to interact with a portion of a nuclear poly(A) polymerase. This interaction was attributable to a 60 amino acid domain in the CPSF100 polypeptide and the N-terminal 220 amino acids of the poly(A) polymerase. An analogous interaction has yet to be described in other eukaryotes. The interaction with PAP thus indicates that the plant CPSF100 polypeptide is likely part of the 3'-end processing machinery, but suggests that this complex may function differently in plants than it does in mammals and yeast.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0167-4412
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
373-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12602868-Amino Acid Sequence, pubmed-meshheading:12602868-Arabidopsis, pubmed-meshheading:12602868-Arabidopsis Proteins, pubmed-meshheading:12602868-Cleavage And Polyadenylation Specificity Factor, pubmed-meshheading:12602868-Cloning, Molecular, pubmed-meshheading:12602868-DNA, Complementary, pubmed-meshheading:12602868-Molecular Sequence Data, pubmed-meshheading:12602868-Nuclear Proteins, pubmed-meshheading:12602868-Phylogeny, pubmed-meshheading:12602868-Polynucleotide Adenylyltransferase, pubmed-meshheading:12602868-Protein Binding, pubmed-meshheading:12602868-Saccharomyces cerevisiae, pubmed-meshheading:12602868-Sequence Alignment, pubmed-meshheading:12602868-Sequence Analysis, DNA, pubmed-meshheading:12602868-Sequence Homology, Amino Acid, pubmed-meshheading:12602868-Two-Hybrid System Techniques
pubmed:year
2003
pubmed:articleTitle
An interaction between an Arabidopsis poly(A) polymerase and a homologue of the 100 kDa subunit of CPSF.
pubmed:affiliation
Plant Physiology/Biochemistry/Molecular Biology Program, Department of Agronomy, University of Kentucky, Lexington, KY 40546-0091, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.