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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-2-25
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pubmed:abstractText |
The ethyl acetate (EA) fraction obtained from a methanol extract of Spatholobi caulis (Leguminosae) has been investigated for anti-metastatic activities in vitro. The EA fraction of Spatholobi caulis inhibited platelet aggregation induced by B16BL6 melanoma cells with an IC(50) of 50 microgram/mL. The EA fraction significantly inhibited HT1080 cancer cell invasion through a matrigel-coated filter with an IC(50) of 25 microgram/mL. Messenger RNA expression of uPA was effectively decreased in HT1080 cells by the EA fraction of Spatholobi caulis with an IC(50) of 30 microgram/mL, but the expressions of MMP-2 (matrix metalloproteinase) and TIMPs (tissue inhibitors of metalloproteinases) were not changed. These findings indicated that the EA fraction suppressed tumour cell invasion by downregulation of uPA (urokinase-type plasminogen activator). Taken together, these results suggest that the EA fraction of Spatholobi caulis may have anti-metastatic activities by blocking tumour cell-induced platelet aggregation (TCIPA) and tumour cell invasion.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0951-418X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2003 John Wiley & Sons, Ltd.
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pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
163-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12601681-Animals,
pubmed-meshheading:12601681-Antineoplastic Agents,
pubmed-meshheading:12601681-DNA Primers,
pubmed-meshheading:12601681-Dose-Response Relationship, Drug,
pubmed-meshheading:12601681-Fabaceae,
pubmed-meshheading:12601681-Fibrosarcoma,
pubmed-meshheading:12601681-Humans,
pubmed-meshheading:12601681-Inhibitory Concentration 50,
pubmed-meshheading:12601681-Male,
pubmed-meshheading:12601681-Matrix Metalloproteinase 2,
pubmed-meshheading:12601681-Melanoma,
pubmed-meshheading:12601681-Mice,
pubmed-meshheading:12601681-Mice, Inbred ICR,
pubmed-meshheading:12601681-Phytotherapy,
pubmed-meshheading:12601681-Plant Extracts,
pubmed-meshheading:12601681-Plant Stems,
pubmed-meshheading:12601681-Platelet Aggregation,
pubmed-meshheading:12601681-RNA, Messenger,
pubmed-meshheading:12601681-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12601681-Tissue Inhibitor of Metalloproteinases,
pubmed-meshheading:12601681-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
Effects of the ethyl acetate fraction of Spatholobi caulis on tumour cell aggregation and migration.
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pubmed:affiliation |
Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University, Yongin 449-701, Korea.
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pubmed:publicationType |
Journal Article
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