Linked Life Data

12600995

Source:http://linkedlifedata.com/resource/pubmed/id/12600995

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
2003-5-5
pubmed:databankReference
pubmed:abstractText
A cDNA encoding a secretory protein hitherto unknown was cloned from mouse adrenocortical cells by subtractive hybridization between the cells without and with expressing steroid 11beta-hydroxylase (Cyp11b-1), a marker for the functional differentiation of cells in the zonae fasciculata reticularis (zFR). The deduced protein consisting of 466 amino acids contained a secretory signal, epidermal growth factor-like repeats, and a proteolytically inactive cathepsin B-related sequence. The amino acid sequence was 89% identical with that of human tubulointerstitial nephritis antigen-related protein. Among the mouse organs examined, adrenal glands prominently expressed its mRNA. The mRNA and its encoded protein were detected in the outer adrenocortical zones that do not express Cyp11b-1, i.e. the zona glomerulosa and the undifferentiated cell zone, while being undetectable in zFR that express Cyp11b-1. The new protein was designated as adrenocortical zonation factor 1 (AZ-1). Clonal lines with different levels of AZ-1 expression were established from Y-1 adrenocortical cells that originally express Cyp11b-1 but little AZ-1. Analyses of the clonal lines revealed that Cyp11b-1 is detected in the clonal lines maintaining little AZ-1 expression and becomes undetectable in those expressing AZ-1. On the other hand, irrespective of the AZ-1 expression, all clones expressed cholesterol side-chain cleavage enzyme, which occurs throughout the cortical zones. These results demonstrated that adrenocortical cells expressing AZ-1 do not express Cyp11b-1, whereas those with little AZ-1 express this zFR marker in vitro and in vivo, implying a putative role of AZ-1 in determining the zonal differentiation of adrenocortical cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
278
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17084-92
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12600995-Adrenal Cortex, pubmed-meshheading:12600995-Amino Acid Sequence, pubmed-meshheading:12600995-Animals, pubmed-meshheading:12600995-Base Sequence, pubmed-meshheading:12600995-Carrier Proteins, pubmed-meshheading:12600995-Cathepsins, pubmed-meshheading:12600995-Cell Differentiation, pubmed-meshheading:12600995-Cloning, Molecular, pubmed-meshheading:12600995-Cysteine, pubmed-meshheading:12600995-Enzyme Precursors, pubmed-meshheading:12600995-Lipocalins, pubmed-meshheading:12600995-Mice, pubmed-meshheading:12600995-Molecular Sequence Data, pubmed-meshheading:12600995-Neoplasm Proteins, pubmed-meshheading:12600995-Protein Biosynthesis, pubmed-meshheading:12600995-Proteins, pubmed-meshheading:12600995-Sequence Alignment, pubmed-meshheading:12600995-Sequence Analysis, Protein, pubmed-meshheading:12600995-Steroid 11-beta-Hydroxylase
pubmed:year
2003
pubmed:articleTitle
An inverse correlation between expression of a preprocathepsin B-related protein with cysteine-rich sequences and steroid 11beta -hydroxylase in adrenocortical cells.
pubmed:affiliation
Department of Biochemistry and Integrative Medical Biology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. mukaik@sc.itc.keio.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't