Linked Life Data

12600899

Source:http://linkedlifedata.com/resource/pubmed/id/12600899

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-3-7
pubmed:abstractText
Oxidative stress may contribute to hypertension. The goals of this study were to determine whether extracellular superoxide dismutase (ECSOD) reduces arterial pressure in spontaneously hypertensive rats (SHR) and whether its heparin-binding domain (HBD), which is responsible for cellular binding, is necessary for the function of ECSOD. Three days after intravenous injection of an adenoviral vector expressing human ECSOD (AdECSOD), mean arterial pressure (MAP) decreased from 165+/-4 mm Hg (mean+/-SE, n=7) to 124+/-3 mm Hg (n=7) in adult anesthetized SHR (P<0.01) but was not altered in normotensive Wistar-Kyoto rats. Cardiac output was not changed in SHR 3 days after AdECSOD. Gene transfer of ECSOD with deletion of the HBD (AdECSODDeltaHBD) had no effect on SHR MAP, even though plasma SOD activity was greater after AdECSODDeltaHBD than after AdECSOD. Immunohistochemistry revealed intense staining for ECSOD in blood vessels and kidneys after AdECSOD but not after AdECSODDeltaHBD. Impaired relaxation of the carotid artery to acetylcholine in SHR was significantly improved after AdECSOD. Cumulative sodium balance in SHR was reduced by AdECSOD compared with AdECSODDeltaHBD. Gene transfer of ECSOD also reduced MAP in conscious SHR, although the effect was not as profound as in anesthetized SHR. In summary, gene transfer of ECSOD, with a strict requirement for its HBD, reduces systemic vascular resistance and arterial pressure in a genetic model of hypertension. This reduction in arterial pressure may be mediated by vasomotor and/or renal mechanisms.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1524-4571
pubmed:author
pubmed:issnType
Electronic
pubmed:day
7
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
461-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12600899-Adenoviridae, pubmed-meshheading:12600899-Anesthesia, pubmed-meshheading:12600899-Animals, pubmed-meshheading:12600899-Binding Sites, pubmed-meshheading:12600899-Blood Pressure, pubmed-meshheading:12600899-Cardiac Output, pubmed-meshheading:12600899-Consciousness, pubmed-meshheading:12600899-Gene Therapy, pubmed-meshheading:12600899-Genetic Vectors, pubmed-meshheading:12600899-Heparin, pubmed-meshheading:12600899-Humans, pubmed-meshheading:12600899-Hypertension, pubmed-meshheading:12600899-Injections, Intravenous, pubmed-meshheading:12600899-Male, pubmed-meshheading:12600899-Mutation, pubmed-meshheading:12600899-Rats, pubmed-meshheading:12600899-Rats, Inbred SHR, pubmed-meshheading:12600899-Rats, Inbred WKY, pubmed-meshheading:12600899-Superoxide Dismutase, pubmed-meshheading:12600899-Time Factors
pubmed:year
2003
pubmed:articleTitle
Gene transfer of extracellular superoxide dismutase reduces arterial pressure in spontaneously hypertensive rats: role of heparin-binding domain.
pubmed:affiliation
Cardiovascular Center and Department of Internal Medicine, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa 52242, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't