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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-2-25
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pubmed:abstractText |
A human anti-B antibody of clone BT97 was obtained from a healthy individual of type A of the ABO blood group without immunization. Cloning was performed by means of heterohybridoma formation of cell fusion between human peripheral lymphocytes and mouse myeloma cells. The antibody selectively reacted with B-antigen in flow cytometry using red blood cells and enzyme-linked immunosorbent assay. The VH and VL genes of BT97 were derived from the germline genes of DP-47 and 3p.81A4, respectively, with a couple of somatic mutational events. Comparative analysis with other reported anti-A, B and H antibodies revealed that the amino acid sequence of the VH region was more homologous than that of the VL region. The sequence of BT97 showed complete identity with one anti-H natural antibody reported by Marks et al., with the exception of the CDR3 region. It is not known whether the homologies include the common properties of the natural antibodies; however, a particular germline gene potentially changes to anti-ABH antibodies. We think that this method is suitable for cDNA preparation of human monoclonal antibodies to blood group antigens and for sequence analysis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0165-2478
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
45-51
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12600744-ABO Blood-Group System,
pubmed-meshheading:12600744-Amino Acid Sequence,
pubmed-meshheading:12600744-Animals,
pubmed-meshheading:12600744-Antibodies, Monoclonal,
pubmed-meshheading:12600744-Antibody Specificity,
pubmed-meshheading:12600744-Cell Fusion,
pubmed-meshheading:12600744-Clone Cells,
pubmed-meshheading:12600744-Complementarity Determining Regions,
pubmed-meshheading:12600744-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12600744-Erythrocytes,
pubmed-meshheading:12600744-Flow Cytometry,
pubmed-meshheading:12600744-Humans,
pubmed-meshheading:12600744-Immunoglobulin Heavy Chains,
pubmed-meshheading:12600744-Immunoglobulin Light Chains,
pubmed-meshheading:12600744-Immunoglobulin Variable Region,
pubmed-meshheading:12600744-Mice,
pubmed-meshheading:12600744-Molecular Sequence Data,
pubmed-meshheading:12600744-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12600744-Structural Homology, Protein
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pubmed:year |
2003
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pubmed:articleTitle |
Molecular characterization of a human monoclonal antibody to B antigen in ABO blood type.
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pubmed:affiliation |
Department of Forensic Medicine, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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