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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2003-10-29
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pubmed:abstractText |
Amplified fragment length polymorphism (AFLP) is a PCR-based technique that involves restriction of genomic DNA followed by ligation of adaptors to the fragments generated and selective PCR amplification of a subset of these fragments. The amplified fragments are separated on a sequencing gel and visualized by autoradiography or fluorescent sequencing equipment. AFLP allows high-resolution genotyping but the lack of a format for databasing and comparison of AFLP fingerprint profiles limits its wider applications in profiling large numbers of biological samples.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1471-2105
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
25
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
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pubmed:year |
2003
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pubmed:articleTitle |
A format for databasing and comparison of AFLP fingerprint profiles.
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pubmed:affiliation |
Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604. hongy@tll.org.sg
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pubmed:publicationType |
Journal Article,
Comparative Study
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