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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-2-24
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pubmed:abstractText |
EPC-1/PEDF expression is closely associated with reversible growth arrest in normal human diploid fibroblast-like (HDF) cells and is diminished with proliferative senescence in vitro. EPC-1 expression in HDF cells is induced under conditions of density-dependent contact inhibition and growth factor deprivation. Antiserum generated against EPC-1 recognizes a secreted protein of approximately 50 kDa from medium conditioned by early passage HDF cells, but not from senescent cells. The addition of EPC-1 antiserum to early population doubling level (PDL) cultures near the plateau phase of growth significantly increases the number of cells entering DNA synthesis. Affinity purified EPC-1 antibodies alone enhance the ability of near plateau-phase early PDL WI-38 cells to synthesize DNA by as much as threefold. Further, the addition of recombinant EPC-1 (rEPC-1) to logarithmically growing cells resulted in a marked decrease in the ability of these cells to enter DNA synthesis. We also demonstrate the loss of EPC-1 expression in WI-38 and IMR-90 HDF cell lines with both senescence and simian virus 40 (SV40) transformation. The loss of EPC-1 expression with SV40 transformation occurs at the level of steady-state mRNA and protein accumulation with genomic EPC-1 sequences grossly intact. Taken together, these results suggest that EPC-1 may play a role in the entry of early passage fibroblasts into a G(0) state or the maintenance of such a state once reached.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Eye Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serpins,
http://linkedlifedata.com/resource/pubmed/chemical/pigment epithelium-derived factor
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9541
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2003 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
195
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
12-20
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12599204-Antibodies,
pubmed-meshheading:12599204-Blotting, Northern,
pubmed-meshheading:12599204-Blotting, Western,
pubmed-meshheading:12599204-Cell Aging,
pubmed-meshheading:12599204-Cell Division,
pubmed-meshheading:12599204-Cell Line,
pubmed-meshheading:12599204-Cell Line, Transformed,
pubmed-meshheading:12599204-Culture Media, Conditioned,
pubmed-meshheading:12599204-DNA,
pubmed-meshheading:12599204-Diploidy,
pubmed-meshheading:12599204-Eye Proteins,
pubmed-meshheading:12599204-Fibroblasts,
pubmed-meshheading:12599204-G0 Phase,
pubmed-meshheading:12599204-Growth Substances,
pubmed-meshheading:12599204-Humans,
pubmed-meshheading:12599204-Immune Sera,
pubmed-meshheading:12599204-Nerve Growth Factors,
pubmed-meshheading:12599204-Proteins,
pubmed-meshheading:12599204-Recombinant Proteins,
pubmed-meshheading:12599204-Serpins,
pubmed-meshheading:12599204-Simian virus 40
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pubmed:year |
2003
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pubmed:articleTitle |
Putative role for EPC-1/PEDF in the G0 growth arrest of human diploid fibroblasts.
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pubmed:affiliation |
Center for Gerontological Research, Medical College of Pennsylvania, Philadelphia, Pennsylvania, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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