Linked Life Data

12597128

Source:http://linkedlifedata.com/resource/pubmed/id/12597128

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-2-24
pubmed:abstractText
The inhibition of vascular smooth muscle cell proliferation is mediated through two intracellular estrogen receptors (ERs), ER-alpha and ER-beta. Deletion variants of ER-alpha have been decribed for cultures of smooth muscle cells. The internal thoracic artery is frequently used as coronary artery bypass graft, yet neither has it been studied for the expression of ER subtypes nor for the synthesis of the ERs in morphologically hetergeneous smooth muscle cells. Using nested RT-PCR, we have demonstrated the mRNA for ER-alpha splicing variants in intact human internal thoracic arteries. The 7A deletion variant occurred in 8 out of 12 cases, the full-length transcript in three cases. The full-length transcript was always found for the ER-beta. Immunolocalization revealed ER-positive nuclei in the desmin-positive subset of smooth muscle cells, but not in cytokeratin (CK)-positive cells of the thickened intima. Morphological evidence is presented suggesting that ER synthesis is high in the tunica media when cell proliferation of smooth muscle cells is increased. We conclude that, in internal thoracic arteries, the 7A deletion variant of ER-a occurs in 75%, whereas the full-length transcript is found in all cases. The significance remains unclear.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0940-9602
pubmed:author
pubmed:issnType
Print
pubmed:volume
185
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
57-65
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12597128-Alternative Splicing, pubmed-meshheading:12597128-Base Sequence, pubmed-meshheading:12597128-DNA Primers, pubmed-meshheading:12597128-Desmin, pubmed-meshheading:12597128-Estrogen Receptor alpha, pubmed-meshheading:12597128-Estrogen Receptor beta, pubmed-meshheading:12597128-Gene Expression Regulation, pubmed-meshheading:12597128-Genetic Variation, pubmed-meshheading:12597128-Humans, pubmed-meshheading:12597128-Immunohistochemistry, pubmed-meshheading:12597128-Mammary Arteries, pubmed-meshheading:12597128-Muscle, Smooth, Vascular, pubmed-meshheading:12597128-Polymerase Chain Reaction, pubmed-meshheading:12597128-RNA, Messenger, pubmed-meshheading:12597128-Receptors, Estrogen, pubmed-meshheading:12597128-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12597128-Transcription, Genetic
pubmed:year
2003
pubmed:articleTitle
Estrogen receptor expression and synthesis in the human internal thoracic artery.
pubmed:affiliation
Institute of Anatomy, University of Leipzig, Liebigstrasse 13, 04103 Leipzig, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't