Linked Life Data

12596572

Source:http://linkedlifedata.com/resource/pubmed/id/12596572

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-2-24
pubmed:abstractText
The Caco-2 cell culture model is widely used during drug development and lead optimization as a predictive tool for the oral absorption of drugs. In order to improve the reliability and quality of the results of Caco-2 experiments and to ensure that the system being used is functionally and enzymatically representative for the intestinal mucosa, it is important to perform a validation of the implemented Caco-2 system. In this paper, we summarize evaluation techniques to guarantee the in-house validity of the model. Theophyllin and sodium fluorescein are used as model compounds to evaluate passive transcellular and passive paracellular transport, respectively. Phenylalanine serves as a substrate to demonstrate active carrier mechanisms. Aminopeptidase and dipeptidyl peptidase are two brush border enzymes present in an active form in the Caco-2 culture model. The presence of an active efflux carrier mechanism is demonstrated with cyclosporin A as a substrate.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0047-2166
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-8
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:articleTitle
Implementation of the caco-2 cell culture model as a predictive tool for the oral absorption of drugs. In-house evaluation procedures.
pubmed:affiliation
Biopharmaceutics & Drug Delivery, Lilly Development Centre, Mont-Saint-Guibert, Belgium.
pubmed:publicationType
Journal Article