Linked Life Data

12595566

Source:http://linkedlifedata.com/resource/pubmed/id/12595566

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2003-2-21
pubmed:abstractText
Cell differentiation and development are highly regulated processes at the transcriptional level. One of the main transcription factors that regulate these processes is AP-2alpha, a cell-type specific protein required for vertebrate development and embryogenesis. AP-2alpha also regulates apoptosis and cell-cycle specific events by interacting with the oncogene c-Myc. In searching for novel AP-2alpha- interacting factors, using an affinity chromatography approach, we have observed that oncoprotein DEK interacts with AP-2alpha in vitro. The existence of an interaction between AP-2alpha and DEK in cellular cultures was demonstrated by expression of a tagged AP-2alpha form followed by immunodetection. By transient co-expression experiments using a reporter for APOE promoter activity we have found that DEK stimulates the transactivation activity of AP-2alpha over APOE promoter. Finally, electrophoretic mobility shift assays suggested that DEK enhances the DNA-binding activity of AP-2alpha. Our data suggest a novel cellular function of DEK as a transcriptional co-activator.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-10037142, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-10446912, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-10519413, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-10694879, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-10837023, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-10899156, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-10966787, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-11018030, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-11320078, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-11522791, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-11694877, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-1509260, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-1549122, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-1989881, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-1989904, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-2041787, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-2792001, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-3040262, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-3063603, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-7504406, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-7525178, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-7555706, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-7753551, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-7846046, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-8622765, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-8622766, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-8660922, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-8808408, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-8922411, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-8989526, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-9050861, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-9256341, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-9271117, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-9425904, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-9468288, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-9566882, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-9632747, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-9776742, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-9811931, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-9830047, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-9858613, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595566-9889285
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1362-4962
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1571-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:12595566-Apolipoproteins E, pubmed-meshheading:12595566-Chromatography, Affinity, pubmed-meshheading:12595566-Chromosomal Proteins, Non-Histone, pubmed-meshheading:12595566-DNA, pubmed-meshheading:12595566-DNA-Binding Proteins, pubmed-meshheading:12595566-Electrophoretic Mobility Shift Assay, pubmed-meshheading:12595566-Humans, pubmed-meshheading:12595566-Oncogene Proteins, pubmed-meshheading:12595566-Promoter Regions, Genetic, pubmed-meshheading:12595566-Protein Binding, pubmed-meshheading:12595566-Recombinant Fusion Proteins, pubmed-meshheading:12595566-Transcription Factor AP-2, pubmed-meshheading:12595566-Transcription Factors, pubmed-meshheading:12595566-Transcriptional Activation, pubmed-meshheading:12595566-Transfection, pubmed-meshheading:12595566-Tumor Cells, Cultured
pubmed:year
2003
pubmed:articleTitle
Transcriptional activation by AP-2alpha is modulated by the oncogene DEK.
pubmed:affiliation
Centro de Biología Molecular Severo Ochoa, CSIC-Universidad Autónoma de Madrid, 28049 Cantoblanco, Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't