Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-2-21
pubmed:abstractText
Listeria monocytogenes is considered as a potential live bacterial vector, particularly for the induction of CD8 T cells. The CD4 T-cell immune response triggered after enteral immunization of mice has not yet been thoroughly characterized. The dynamics of gamma interferon (IFN-gamma)- and interleukin-4 (IL-4)-secreting CD4 T cells were analyzed after priming through intragastric delivery of an attenuated delta actA recombinant L. monocytogenes strain expressing the Leishmania major LACK protein; a peptide of this protein, LACK(158-173) peptide (pLACK), is a well-characterized CD4 T-cell target in BALB/c mice. Five compartments were monitored: Peyer's patches, mesenteric lymph nodes (MLN), spleen, liver, and blood. A single intragastric inoculation of delta actA-LACK-LM in BALB/c mice led to colonization of the MLN and spleen at a significant level for at least 3 days. Efficient priming of IFN-gamma-secreting pLACK-reactive CD4 T cells was observed in all tested compartments. Interestingly, IL-4-secreting pLACK-reactive CD4 T cells were detectable at day 6 or 7 only in blood and liver. The absence of translocation of viable bacteria through the intestinal epithelium after further delta actA-LACK-LM inoculations was concomitant with the absence of an increase in the level of IFN-gamma secreted by the MLN, blood, and splenic pLACK-reactive Th1 T cells, although the levels remained significantly above the basal level. No change in this population size was detected in the spleen. However, an increase in the number of intragastric inoculations had a clinical beneficial effect in L. major-infected BALB/c mice. L. monocytogenes thus presents the potential of an efficient vector for induction of CD4 T cells when administered by the enteral route.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-10092078, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-10415046, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-10781863, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-11018170, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-11067920, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-11094295, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-11160234, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-11207297, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-11222702, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-11298655, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-11781372, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-11970995, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-12034096, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-12055256, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-1286064, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-1409724, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-1682378, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-1739966, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-1943998, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-2202764, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-2831066, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-6431408, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-6772561, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-7546656, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-7725103, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-7732018, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-7790091, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-8832890, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-9116648, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-9141215, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-9175832, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-9209512, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-9314077, http://linkedlifedata.com/resource/pubmed/commentcorrection/12595418-9453636
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1083-90
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Enteral immunization with attenuated recombinant Listeria monocytogenes as a live vaccine vector: organ-dependent dynamics of CD4 T lymphocytes reactive to a Leishmania major tracer epitope.
pubmed:affiliation
Unité d'Immunophysiologie et Parasitisme Intracellulaire, Institut Pasteur, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't