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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2003-3-5
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pubmed:abstractText |
RNA interference represents an exciting new technology that could have therapeutic applications for the treatment of viral infections. Hepatitis C virus (HCV) is a major cause of chronic liver disease and affects >270 million individuals worldwide. The HCV genome is a single-stranded RNA that functions as both a messenger RNA and replication template, making it an attractive target for the study of RNA interference. Double-stranded small interfering RNA (siRNA) molecules designed to target the HCV genome were introduced through electroporation into a human hepatoma cell line (Huh-7) that contained an HCV subgenomic replicon. Two siRNAs dramatically reduced virus-specific protein expression and RNA synthesis to levels that were 90% less than those seen in cells treated with negative control siRNAs. These same siRNAs protected naive Huh-7 cells from challenge with HCV replicon RNA. Treatment of cells with synthetic siRNA was effective >72 h, but the duration of RNA interference could be extended beyond 3 weeks through stable expression of complementary strands of the interfering RNA by using a bicistronic expression vector. These results suggest that a gene-therapeutic approach with siRNA could ultimately be used to treat HCV.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12594341-10390360,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/12594341-9228008
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
100
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2783-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12594341-Antibodies, Monoclonal,
pubmed-meshheading:12594341-Blotting, Northern,
pubmed-meshheading:12594341-Blotting, Western,
pubmed-meshheading:12594341-Cell Line,
pubmed-meshheading:12594341-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12594341-Electroporation,
pubmed-meshheading:12594341-Genetic Vectors,
pubmed-meshheading:12594341-Hepatitis C,
pubmed-meshheading:12594341-Humans,
pubmed-meshheading:12594341-Liver,
pubmed-meshheading:12594341-Models, Genetic,
pubmed-meshheading:12594341-Mutation,
pubmed-meshheading:12594341-Plasmids,
pubmed-meshheading:12594341-RNA,
pubmed-meshheading:12594341-RNA, Messenger,
pubmed-meshheading:12594341-RNA, Small Interfering,
pubmed-meshheading:12594341-RNA, Viral,
pubmed-meshheading:12594341-RNA Interference,
pubmed-meshheading:12594341-Time Factors,
pubmed-meshheading:12594341-Transcription, Genetic,
pubmed-meshheading:12594341-Transfection,
pubmed-meshheading:12594341-Tumor Cells, Cultured,
pubmed-meshheading:12594341-Virus Replication
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pubmed:year |
2003
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