Source:http://linkedlifedata.com/resource/pubmed/id/12594306
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2003-2-20
|
| pubmed:abstractText |
Macrophages have been proposed as the major effector cell in T cell-mediated xenograft rejection. To determine their role in this response, NOD-SCID mice were transplanted with fetal pig pancreas (FPP) before reconstitution with CD4(+) T cells from BALB/c mice. Twelve days after CD4(+) T cell reconstitution, purified macrophages (depleted of T cells) were isolated from CD4(+) T cell-reconstituted FPP recipient mice and adoptively transferred to their nonreconstituted counterparts. After adoptive macrophage transfer, FPP recipient mice transferred with macrophages from CD4(+) T cell-reconstituted mice demonstrated xenograft destruction along with massive macrophage infiltration at day 4 and complete graft destruction at day 8 postmacrophage transfer. By contrast, FPP recipients that received macrophages from nonreconstituted mice showed intact FPP xenografts with few infiltrating macrophages at both days 4 and 8 after macrophage transfer. The graft-infiltrating macrophages showed increased expression of their activation markers. Depletion of endogenous macrophages or any remaining CD4(+) T cells did not delay graft rejection in the macrophage-transferred FPP recipients, whereas depletion of transferred macrophages with clodronate liposomes prevented graft rejection. Our results show that macrophages primed by FPP and activated by CD4(+) T cells were attracted from the peripheral circulation and were capable of specific targeting and destruction of FPP xenografts. This suggests that in xenograft rejection, there are macrophage-specific recognition and targeting signals that are independent of those received by T cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0022-1767
|
| pubmed:author |
pubmed-author:ChandraAbhilashA,
pubmed-author:DaveyKellyK,
pubmed-author:HawthorneWayne JWJ,
pubmed-author:KOCKN GNG,
pubmed-author:LehnertAnne MAM,
pubmed-author:O'ConnellPhilip JPJ,
pubmed-author:PatelAnita TAT,
pubmed-author:WaltersStacey NSN,
pubmed-author:WongJeferey Kwok WahJK,
pubmed-author:YiShounanS,
pubmed-author:van RooijenNicoN
|
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
170
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2750-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12594306-Adoptive Transfer,
pubmed-meshheading:12594306-Animals,
pubmed-meshheading:12594306-Biological Markers,
pubmed-meshheading:12594306-CD4-Positive T-Lymphocytes,
pubmed-meshheading:12594306-Cell Movement,
pubmed-meshheading:12594306-Cytotoxicity, Immunologic,
pubmed-meshheading:12594306-Female,
pubmed-meshheading:12594306-Fetal Tissue Transplantation,
pubmed-meshheading:12594306-Graft Rejection,
pubmed-meshheading:12594306-Immunophenotyping,
pubmed-meshheading:12594306-Islets of Langerhans Transplantation,
pubmed-meshheading:12594306-Macrophage Activation,
pubmed-meshheading:12594306-Macrophages,
pubmed-meshheading:12594306-Male,
pubmed-meshheading:12594306-Mice,
pubmed-meshheading:12594306-Mice, Inbred BALB C,
pubmed-meshheading:12594306-Mice, Inbred NOD,
pubmed-meshheading:12594306-Mice, SCID,
pubmed-meshheading:12594306-Signal Transduction,
pubmed-meshheading:12594306-Swine,
pubmed-meshheading:12594306-T-Lymphocyte Subsets,
pubmed-meshheading:12594306-Transplantation, Heterologous,
pubmed-meshheading:12594306-Transplantation Conditioning
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
T cell-activated macrophages are capable of both recognition and rejection of pancreatic islet xenografts.
|
| pubmed:affiliation |
National Pancreas Transplant Unit, University of Sydney at Westmead Hospital, Westmead, New South Wales, Australia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|