Source:http://linkedlifedata.com/resource/pubmed/id/12594301
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2003-2-20
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| pubmed:abstractText |
Transplantation of HLA-identical or haploidentical T cell-depleted allogeneic bone marrow (BM) into SCID infants results in thymus-dependent T cell development in the recipients. Immunoscope analysis of the TCR V beta repertoire was performed on 15 SCID patients given BM transplants. Before and within the first 100 days after bone marrow transplantation (BMT), patients' PBMC displayed an oligoclonal or skewed T cell repertoire, low TCR excision circles (TREC) values, and a predominance of CD45RO(+) T cells. In contrast, the presence of high numbers of CD45RA(+) cells in the circulation of SCID patients >100 days post-BMT correlated with active T cell output by the thymus as revealed by high TREC values and a polyclonal T cell repertoire demonstrated by a Gaussian distribution of V beta-specific peaks. Ten years after BMT, we observed a decrease of the normal polyclonal T cell repertoire and an increase of a more skewed T cell repertoire. A decline of TREC levels and a decrease in the number of CD45RA(+) cells beyond 10 years after BMT was concomitant with the detection of oligoclonal CD3(+)CD8(+)CD45RO(+) cells. The switch from a polyclonal to a more skewed repertoire, observed in the CD3(+)CD8(+)CD45RO(+) T cell subset, is a phenomenon that occurs normally with decreased thymic output during aging, but not as rapidly as in this patient population. We conclude that a normal T cell repertoire develops in SCID patients as a result of thymic output and the repertoire remains highly diverse for the first 10 years after BMT. The TCR diversity positively correlates in these patients with TREC levels.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
170
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2711-8
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12594301-Antigens, CD45,
pubmed-meshheading:12594301-Bone Marrow Transplantation,
pubmed-meshheading:12594301-CD8-Positive T-Lymphocytes,
pubmed-meshheading:12594301-Cell Differentiation,
pubmed-meshheading:12594301-Cell Division,
pubmed-meshheading:12594301-Clone Cells,
pubmed-meshheading:12594301-Complementarity Determining Regions,
pubmed-meshheading:12594301-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:12594301-Humans,
pubmed-meshheading:12594301-Immunophenotyping,
pubmed-meshheading:12594301-Leukocytes, Mononuclear,
pubmed-meshheading:12594301-Longitudinal Studies,
pubmed-meshheading:12594301-Lymphocyte Count,
pubmed-meshheading:12594301-Lymphopenia,
pubmed-meshheading:12594301-Postoperative Period,
pubmed-meshheading:12594301-Preoperative Care,
pubmed-meshheading:12594301-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:12594301-Severe Combined Immunodeficiency,
pubmed-meshheading:12594301-T-Lymphocyte Subsets,
pubmed-meshheading:12594301-Thymus Gland
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| pubmed:year |
2003
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| pubmed:articleTitle |
T cell repertoire development in humans with SCID after nonablative allogeneic marrow transplantation.
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| pubmed:affiliation |
Department of Immunology, Medicine, and Pediatrics, Duke University Medical Center, Durham, NC 27710, USA. msarzott@duke.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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