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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2003-2-19
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pubmed:abstractText |
Neuropeptide Y (NPY) plays an important role in the central and sympathetic regulation of food intake and blood pressure. Although the NPY gene expression is regulated by a number of agents such as leptin, the mechanism responsible for leptin-induced regulation of the transcription of the NPY gene remains to be explored. In this study, the NPY gene promoter was transactivated by leptin in N18TG2, NG108-15 and PC12 cells which expressed the functional leptin receptor. The long isoform of leptin receptor (OB-Rb) could induce the transactivation, but the C-terminal truncated form (OB-Ra) could not. When dominant negative type of STAT3, JAK1 or JAK2 and was co-expressed, the leptin-induced transactivation was suppressed almost completely. The leptin-response element which confers NPY gene transactivation by leptin was determined in the 221-bp region of rat NPY gene promoter (-553/-335), where two STAT3-binding site-like elements (TCCAGTA) exist. These results indicated that activation of JAK1, JAK2 and STAT3 is necessary for leptin-induced transactivation of NPY gene through the leptin-response element in these neural cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/JAK1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/JAK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Jak1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Jak1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Jak2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Jak2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Janus Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Janus Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Leptin,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptide Y,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0197-0186
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
591-601
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12590942-Animals,
pubmed-meshheading:12590942-Base Sequence,
pubmed-meshheading:12590942-DNA-Binding Proteins,
pubmed-meshheading:12590942-Electrophysiology,
pubmed-meshheading:12590942-Humans,
pubmed-meshheading:12590942-Janus Kinase 1,
pubmed-meshheading:12590942-Janus Kinase 2,
pubmed-meshheading:12590942-Leptin,
pubmed-meshheading:12590942-Mice,
pubmed-meshheading:12590942-Molecular Sequence Data,
pubmed-meshheading:12590942-Neurons,
pubmed-meshheading:12590942-Neuropeptide Y,
pubmed-meshheading:12590942-Promoter Regions, Genetic,
pubmed-meshheading:12590942-Protein-Tyrosine Kinases,
pubmed-meshheading:12590942-Proto-Oncogene Proteins,
pubmed-meshheading:12590942-Rats,
pubmed-meshheading:12590942-Response Elements,
pubmed-meshheading:12590942-STAT3 Transcription Factor,
pubmed-meshheading:12590942-Signal Transduction,
pubmed-meshheading:12590942-Trans-Activators,
pubmed-meshheading:12590942-Transcriptional Activation,
pubmed-meshheading:12590942-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
Leptin-induced transactivation of NPY gene promoter mediated by JAK1, JAK2 and STAT3 in the neural cell lines.
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pubmed:affiliation |
Division of Pharmacology, Department of Molecular Genetics, Course for Molecular and Cellular Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, Niigata 951 8510, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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