Linked Life Data

12587874

Source:http://linkedlifedata.com/resource/pubmed/id/12587874

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-2-17
pubmed:databankReference
pubmed:abstractText
Herbicide safeners are known to protect monocotyledonous crops from herbicide injury by accelerating the metabolism of herbicides. We have investigated the effects of the safener cloquintocetmexyl, which protects small-grain cereals against the graminicidal herbicide, clodinafop-propargyl. Subtractive suppression hybridisation was used to identify wheat genes which are up-regulated by treatment not only with cloquintocet-mexyl but also with phenobarbital, which is known to stimulate xenobiotic metabolism in animals and plants. DNA sequences of five glutathione transferases (GSTs) belonging to three different classes and a multidrug resistance associated protein (MRP) homologue were identified in the screen. The chemical inducibility of these clones was confirmed by Northern analysis. The MRP protein was shown to be induced by treatments with cloquintocet-mexyl and phenobarbital and to be localised to the tonoplast. Since clodinafop-propargyl is not known to be metabolised by glutathionylation, the significance of GST induction is interpreted in terms of a generalised response to chemical stress, particularly the generation of active oxygen species. This work establishes herbicide safeners as useful tools for the identification of genes encoding herbicide-metabolising enzymes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1526-498X
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
202-14
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12587874-Amino Acid Sequence, pubmed-meshheading:12587874-Blotting, Western, pubmed-meshheading:12587874-Cloning, Molecular, pubmed-meshheading:12587874-DNA, Complementary, pubmed-meshheading:12587874-Gene Expression Regulation, Enzymologic, pubmed-meshheading:12587874-Gene Expression Regulation, Plant, pubmed-meshheading:12587874-Glutathione Transferase, pubmed-meshheading:12587874-Isoenzymes, pubmed-meshheading:12587874-Molecular Sequence Data, pubmed-meshheading:12587874-Multidrug Resistance-Associated Proteins, pubmed-meshheading:12587874-Phenobarbital, pubmed-meshheading:12587874-Phylogeny, pubmed-meshheading:12587874-Sequence Alignment, pubmed-meshheading:12587874-Sequence Analysis, DNA, pubmed-meshheading:12587874-Sequence Homology, Amino Acid, pubmed-meshheading:12587874-Triticum, pubmed-meshheading:12587874-Xenobiotics
pubmed:year
2003
pubmed:articleTitle
Co-induction of glutathione-S-transferases and multidrug resistance associated protein by xenobiotics in wheat.
pubmed:affiliation
Crop Performance and Improvement Division, IACR-Rothamsted, Harpenden AL5 2JQ, UK. freddie.theodoulou@bbsrc.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't